PRODUCT CODE: ET1704-23

Recombinant FAP1 Monoclonal Antibody (ET1704-23)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of FAP1 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-23, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of FAP1 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-23, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-FAP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-23, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of FAP1 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-23, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant FAP1 Monoclonal Antibody (ET1704-23)

Immunogen

Recombinant protein within c-terminal of human fap1 aa 1-120.

Host

Rabbit

Positive Control

Hela cell lysates, human colon carcinoma tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA56-11

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

95 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

FAP1

SYNONYMS

APO1/CD95 (Fas) associated phosphatase antibody; DKFZp686J1497 antibody; FAP 1 antibody; FAP1 antibody; Fas associated phosphatase 1 antibody; Fas associated protein tyrosine phosphatase 1 antibody; HGNC:9646 antibody; hPTPE1 antibody; Phosphatase rip antibody; PNP 1 antibody; PNP1 antibody; Protein tyrosine phosphatase 1 Fas associated antibody; protein tyrosine phosphatase 1E antibody; Protein tyrosine phosphatase non receptor type 13 antibody; Protein tyrosine phosphatase nonreceptor type 13 antibody; Protein tyrosine phosphatase PTPL1 antibody; protein tyrosine phosphatase, non-receptor type 13 (APO-1/CD95 (Fas)-associated phosphatase) antibody; PTP BAS antibody; PTP BL antibody; PTP E1 antibody; PTP1E antibody; PTPL 1 antibody; PTPL1 antibody; PTPLE antibody; PTPN 13 antibody; PTPN13 antibody; Tyrosine protein phosphatase non receptor type 13 antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase S9B family.

TISSUE SPECIFICITY

Expressed in adipose tissue. Expressed in the dermal fibroblasts in the fetal skin. Expressed in the granulation tissue of healing wounds and on reactive stromal fibroblast in epithelial cancers. Expressed in activated fibroblast-like synoviocytes from inflamed synovial tissues. Expressed in activated hepatic stellate cells (HSC) and myofibroblasts from cirrhotic liver, but not detected in normal liver. Expressed in glioma cells (at protein level). Expressed in glioblastomas and glioma cells. Isoform 1 and isoform 2 are expressed in melanoma, carcinoma and fibroblast cell lines.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated.; The N-terminus may be blocked.

SUBCELLULAR LOCATION

Cell membrane. Cell surface.

FUNCTION

FAP (fibroblast activation protein) is a cell surface glycoprotein and serine protease that is expressed primarily in fetal mesenchymal tissues and epithelial cancer fibroblasts. In cancer, FAP functions to promote cellular proliferation. In embryonic development, FAP functions to remodel developing tissues. FAP acts as an integral membrane gelatinase composed of N-glycosylated proteolytically inactive subunits. FAP expression on chondrocyte membranes is upregulated by the combination of the cytokines IL-1 and OSM and has been shown to increase in osteoarthritic patients. This expression is co-localized with MMP-1and MMP-13 as well as CD44 (variants v3 and v7/8). Mice that lack all copies of the FAP gene have been found to be fertile and to have developmental defects or change in cancer susceptibility.