PRODUCT CODE: ET1703-25

Recombinant Ezrin Monoclonal Antibody (ET1703-25)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of Ezrin on different cell lysates using anti-Ezrin antibody at 1/1,000 dilution.<br />
Positive control:<br />
Lane 1: Hela<br />
Lane 2: Jurkat<br />
Lane 3: A431
  • Western blot analysis of Ezrin on different cell lysates using anti-Ezrin antibody at 1/1,000 dilution.<br />
Positive control:<br />
Lane 1: Hela<br />
Lane 2: Jurkat<br />
Lane 3: A431
  • Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Ezrin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Ezrin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Ezrin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Ezrin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Ezrin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Ezrin antibody. Counter stained with hematoxylin.
Western blot analysis of Ezrin on different cell lysates using anti-Ezrin antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Jurkat
Lane 3: A431

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Ezrin Monoclonal Antibody (ET1703-25)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, Jurkat, A431, human lung tissue, human lung cancer tissue, human liver cancer tissue, human colon cancer tissue, human breast carcinoma tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-65

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

80 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Ezrin

GENE NAME

EZR

SYNONYMS

EZR

TISSUE SPECIFICITY

Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.

DEVELOPMENTAL STAGE

Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding (By similarity).; S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

SUBCELLULAR LOCATION

Apical cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection. Cell projection, microvillus membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cell projection, microvillus. Note=Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein (cytoplasmic side).

FUNCTION

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.