PRODUCT CODE: ET1705-23

Recombinant ETS1 Monoclonal Antibody (ET1705-23)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of ETS1 on different cell using anti-ETS1 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Line1: Jurkat <br />
Line2: K562 <br />
Line3: HepG2
  • Western blot analysis of ETS1 on different cell using anti-ETS1 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Line1: Jurkat <br />
Line2: K562 <br />
Line3: HepG2
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ETS1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ETS1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-ETS1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with ETS1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of ETS1 on different cell using anti-ETS1 antibody at 1/1,000 dilution.
Positive control:
Line1: Jurkat
Line2: K562
Line3: HepG2

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant ETS1 Monoclonal Antibody (ET1705-23)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HepG2, Jurkat, K562.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM92-32

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Protein C-ets-1

GENE NAME

ETS1

SYNONYMS

ETS1, EWSR2

SEQUENCE SIMILARITIES

Belongs to the ETS family.

TISSUE SPECIFICITY

Highly expressed within lymphoid cells. Isoforms c-ETS-1A and Ets-1 p27 are both detected in all fetal tissues tested, but vary with tissue type in adult tissues. None is detected in brain or kidney.

POST-TRANSLATIONAL MODIFICATION

Sumoylated on Lys-15 and Lys-227, preferentially with SUMO2; which inhibits transcriptional activity.; Ubiquitinated; which induces proteasomal degradation.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus. Note=Delocalizes from nucleus to cytoplasm when coexpressed with isoform Ets-1 p27.

FUNCTION

Transcription factor. Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts. May control the differentiation, survival and proliferation of lymphoid cells. May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion.