PRODUCT CODE: ET1701-69

Recombinant Estrogen Receptor beta Monoclonal Antibody (ET1701-69)

  • Recombinant

Applications

  • WB

REACTIVITY

  • Human

Western blot analysis of Estrogen Receptor beta on 293T cells lysates using anti-Estrogen Receptor beta antibody at 1/1,000 dilution.
  • Western blot analysis of Estrogen Receptor beta on 293T cells lysates using anti-Estrogen Receptor beta antibody at 1/1,000 dilution.
Western blot analysis of Estrogen Receptor beta on 293T cells lysates using anti-Estrogen Receptor beta antibody at 1/1,000 dilution.

Applications

  • WB

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Estrogen Receptor beta Monoclonal Antibody (ET1701-69)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293T, human breast carcinoma tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ09-02

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

59 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

Estrogen receptor beta

GENE NAME

ESR2

SYNONYMS

ESR2, ER-beta

SEQUENCE SIMILARITIES

Belongs to the nuclear hormone receptor family. NR3 subfamily.

TISSUE SPECIFICITY

Isoform 1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform 2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform 4 is found in testis. Isoform 5 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform 6 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-87 and Ser-105 recruits NCOA1.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.