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PRODUCT CODE: ET1705-85

Recombinant Estrogen Inducible Protein pS2 Monoclonal Antibody (ET1705-85)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

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ICC staining Estrogen Inducible Protein pS2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Estrogen Inducible Protein pS2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Estrogen Inducible Protein pS2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Estrogen Inducible Protein pS2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Estrogen Inducible Protein pS2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Estrogen Inducible Protein pS2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MCF-7 cells with Estrogen Inducible Protein pS2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
ICC staining Estrogen Inducible Protein pS2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Estrogen Inducible Protein pS2 Monoclonal Antibody (ET1705-85)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A549, MCF-7, PC-3M, human breast cancer tissue, human stomach cancer tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM37-86

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

Isotype

IgG

APPLICATION DILUTION

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50 WB

TARGET

UNIPROT #

P04155

PROTEIN NAME

Estrogen Inducible Protein pS2

SYNONYMS

BCEI antibody; Breast cancer estrogen inducible protein antibody; Breast cancer estrogen inducible sequence antibody; Breast cancer estrogen-inducible protein antibody; D21S21 antibody; Gastrointestinal trefoil protein antibody; Gastrointestinal trefoil protein pS2 antibody; hP1.A antibody; HP1A antibody; HPS 2 antibody; HPS2 antibody; pNR 2 antibody; PNR-2 antibody; pNR2 antibody; Polypeptide P1.A antibody; Protein pS2 antibody; PS 2 antibody; pS2 antibody; pS2 protein antibody; TFF 1 antibody; TFF1 antibody; TFF1_HUMAN antibody; Trefoil factor 1 antibody

TISSUE SPECIFICITY

Found in stomach, with highest levels in the upper gastric mucosal cells (at protein level). Detected in goblet cells of the small and large intestine and rectum, small submucosal glands in the esophagus, mucous acini of the sublingual gland, submucosal glands of the trachea, and epithelial cells lining the exocrine pancreatic ducts but not in the remainder of the pancreas (at protein level). Scattered expression is detected in the epithelial cells of the gallbladder and submucosal glands of the vagina, and weak expression is observed in the bronchial goblet cells of the pseudostratified epithelia in the respiratory system (at protein level). Detected in urine (at protein level). Strongly expressed in breast cancer but at low levels in normal mammary tissue. It is regulated by estrogen in MCF-7 cells. Strong expression found in normal gastric mucosa and in the regenerative tissues surrounding ulcerous lesions of gastrointestinal tract, but lower expression found in gastric cancer (at protein level).

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain. pS2, also designated Md2, BCEI, TFF1 and pNR-2, is a trefoil protein that is highly expressed in normal gastric mucosa. pS2 has also been detected in a number of carcinomas including cancers of the breast, pancreas and stomach. pS2 is not present in normal breast tissue but is inducible by estrogen in MCF7 cells. pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. The gene encoding pS2 maps to human chromosome 21q22.3.