PRODUCT CODE: ET1604-16

Recombinant ERK1 Monoclonal Antibody (ET1604-16)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: Jurkat <br />
 Lane 3: K562
  • Western blot analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: Jurkat <br />
 Lane 3: K562
  • ICC staining ERK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ERK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ERK1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-ERK1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ERK1 antibody. Counter stained with hematoxylin.
Western blot analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Jurkat
Lane 3: K562

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant ERK1 Monoclonal Antibody (ET1604-16)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat, K562, Hela, A431, NIH/3T3, human kidney tissue, mouse stomach tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SP05-09

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

43 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

ERK1

SYNONYMS

ERK 1 antibody; ERK antibody; ERK-1 antibody; ERK1 antibody; ERT 2 antibody; ERT2 antibody; Extracellular Signal Regulated Kinase 1 antibody; Extracellular signal related kinase 1 antibody; Extracellular signal-regulated kinase 1 antibody; HGNC6877 antibody; HS44KDAP antibody; HUMKER1A antibody; Insulin Stimulated MAP2 Kinase antibody; Insulin-stimulated MAP2 kinase antibody; MAP kinase 1 antibody; MAP kinase 3 antibody; MAP Kinase antibody; MAP kinase isoform p44 antibody; MAPK 1 antibody; MAPK 3 antibody; MAPK antibody; MAPK1 antibody; Mapk3 antibody; MGC20180 antibody; Microtubule Associated Protein 2 Kinase antibody; Microtubule-associated protein 2 kinase antibody; Mitogen Activated Protein Kinase 3 antibody; Mitogen-activated protein kinase 1 antibody; Mitogen-activated protein kinase 3 antibody; MK03_HUMAN antibody; OTTHUMP00000174538 antibody; OTTHUMP00000174541 antibody; p44 ERK1 antibody; p44 MAPK antibody; p44-ERK1 antibody; p44-MAPK antibody; P44ERK1 antibody; P44MAPK antibody; PRKM 3 antibody; PRKM3 antibody; Protein Kinase Mitogen Activated 3 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

ERK 1 antibody ERK antibody ERK-1 antibody ERK1 antibody ERT 2 antibody ERT2 antibody Extracellular Signal Regulated Kinase 1 antibody Extracellular signal related kinase 1 antibody Extracellular signal-regulated kinase 1 antibody HGNC6877 antibody HS44KDAP antibody HUMKER1A antibody Insulin Stimulated MAP2 Kinase antibody Insulin-stimulated MAP2 kinase antibody MAP kinase 1 antibody MAP kinase 3 antibody MAP Kinase antibody MAP kinase isoform p44 antibody MAPK 1 antibody MAPK 3 antibody MAPK antibody MAPK1 antibody Mapk3 antibody MGC20180 antibody Microtubule Associated Protein 2 Kinase antibody Microtubule-associated protein 2 kinase antibody Mitogen Activated Protein Kinase 3 antibody Mitogen-activated protein kinase 1 antibody Mitogen-activated protein kinase 3 antibody MK03_HUMAN antibody OTTHUMP00000174538 antibody OTTHUMP00000174541 antibody p44 ERK1 antibody p44 MAPK antibody p44-ERK1 antibody p44-MAPK antibody P44ERK1 antibody P44MAPK antibody PRKM 3 antibody PRKM3 antibody Protein Kinase Mitogen Activated 3 antibody