PRODUCT CODE: ET1705-33

Recombinant Ephrin B2 Monoclonal Antibody (ET1705-33)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Ephrin B2 on different tissue lysate using anti-Ephrin B2 antibody at 1/1,000 dilution.<br />
Positive control:<br />
 Lane 1: Human kidney<br />
 Lane 2: Mouse lung <br />
 Lane 3: Rat brain<br />
  • Western blot analysis of Ephrin B2 on different tissue lysate using anti-Ephrin B2 antibody at 1/1,000 dilution.<br />
Positive control:<br />
 Lane 1: Human kidney<br />
 Lane 2: Mouse lung <br />
 Lane 3: Rat brain<br />
  • ICC staining Ephrin B2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Ephrin B2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of Ephrin B2 on different tissue lysate using anti-Ephrin B2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Human kidney
Lane 2: Mouse lung
Lane 3: Rat brain

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Ephrin B2 Monoclonal Antibody (ET1705-33)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human kidney, mouse lung, rat brain, Hela, 293T.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM53-21

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Ephrin-B2

GENE NAME

EFNB2

SYNONYMS

LERK-5, HTK-L, EFNB2, EPLG5, HTKL, LERK5

SEQUENCE SIMILARITIES

Belongs to the ephrin family.

TISSUE SPECIFICITY

Lung and kidney.

POST-TRANSLATIONAL MODIFICATION

Inducible phosphorylation of tyrosine residues in the cytoplasmic domain.

SUBCELLULAR LOCATION

Cell membrane; Single-pass type I membrane protein. Cell junction, adherens junction.

FUNCTION

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.; (Microbial infection) Acts as a receptor for Hendra virus and Nipah virus.