PRODUCT CODE: ET1705-79

Recombinant Epac1 Monoclonal Antibody (ET1705-79)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Epac1 on mouse kidney tissue lysate using anti-Epac1 antibody at 1/1,000 dilution.
  • Western blot analysis of Epac1 on mouse kidney tissue lysate using anti-Epac1 antibody at 1/1,000 dilution.
  • Western blot analysis of Epac1 on PC3 cell lysates using anti-Epac1 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Epac1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Epac1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Epac1 antibody. Counter stained with hematoxylin.
Western blot analysis of Epac1 on mouse kidney tissue lysate using anti-Epac1 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • IP

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Epac1 Monoclonal Antibody (ET1705-79)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse kidney tissue lysate, human prostate tissue, human stomach cancer tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM63-29

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:10-1:50 ICC/IF

TARGET

UNIPROT #

PROTEIN NAME

Rap guanine nucleotide exchange factor 3

GENE NAME

RAPGEF3

SYNONYMS

EPAC 1, cAMP-GEFI, RAPGEF3, CGEF1, EPAC, EPAC1

TISSUE SPECIFICITY

Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney.

SUBCELLULAR LOCATION

Endomembrane system.

FUNCTION

Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin.