PRODUCT CODE: ET1705-56

Recombinant ENO1 Monoclonal Antibody (ET1705-56)

  • Zebrafish
  • Recombinant

Applications

  • WB

  • IP

  • FC

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

Western blot analysis of ENO1 on different lysates using anti-ENO1 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Rat brain tissue <br />
 Lane 2: NIH-3T3<br />
 Lane 3: Hela <br />
 Lane 4: HepG2<br />
  • Western blot analysis of ENO1 on different lysates using anti-ENO1 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Rat brain tissue <br />
 Lane 2: NIH-3T3<br />
 Lane 3: Hela <br />
 Lane 4: HepG2<br />
  • Flow cytometric analysis of MCF-7 cells with ENO1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
  • Western blot analysis of ENO1 on Zebrafish tissue lysates using anti-ENO1 antibody at 1/1,000 dilution.
Western blot analysis of ENO1 on different lysates using anti-ENO1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Rat brain tissue
Lane 2: NIH-3T3
Lane 3: Hela
Lane 4: HepG2

Applications

  • WB

  • IP

  • FC

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant ENO1 Monoclonal Antibody (ET1705-56)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat brain tissue lysate, NIH-3T3 cell lysate, Zebrafish tissue lysates, HepG2 cell lysate, Hela cell lysate, MCF-7.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM63-53

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

47 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • FC

  • 1:50-1:100

  • ICC

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

Alpha-enolase

GENE NAME

ENO1

SYNONYMS

NNE, ENO1, ENO1L1, MBPB1, MPB1

SEQUENCE SIMILARITIES

Belongs to the enolase family.

TISSUE SPECIFICITY

The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

DEVELOPMENTAL STAGE

During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.

POST-TRANSLATIONAL MODIFICATION

ISGylated.; Lysine 2-hydroxyisobutyrylation (Khib) by p300/EP300 activates the phosphopyruvate hydratase activity.

SUBCELLULAR LOCATION

Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.; [Isoform MBP-1]: Nucleus.

FUNCTION

Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.; MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.