PRODUCT CODE: ET1703-79

Recombinant Endothelin B Receptor Monoclonal Antibody (ET1703-79)

  • Zebrafish
  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Zebrafish

Western blot analysis of Endothelin B Receptor on different cells lysates using anti-Endothelin B Receptor antibody at 1/500 dilution.<br />
Positive control:<br />
Lane 1: Zebrafish<br />
Lane 2: Raji<br />
Line 3: JAR
  • Western blot analysis of Endothelin B Receptor on different cells lysates using anti-Endothelin B Receptor antibody at 1/500 dilution.<br />
Positive control:<br />
Lane 1: Zebrafish<br />
Lane 2: Raji<br />
Line 3: JAR
Western blot analysis of Endothelin B Receptor on different cells lysates using anti-Endothelin B Receptor antibody at 1/500 dilution.
Positive control:
Lane 1: Zebrafish
Lane 2: Raji
Line 3: JAR

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Zebrafish

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Endothelin B Receptor Monoclonal Antibody (ET1703-79)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

JAR, Raji.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM74-10

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

Endothelin receptor type B

GENE NAME

EDNRB

SEQUENCE SIMILARITIES

Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRB sub-subfamily.

TISSUE SPECIFICITY

Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells.

POST-TRANSLATIONAL MODIFICATION

Palmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405.

SUBCELLULAR LOCATION

Cell membrane; Multi-pass membrane protein. Note=internalized after activation by endothelins.

FUNCTION

Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.