PRODUCT CODE: ET7106-77

Recombinant Endo G Monoclonal Antibody (ET7106-77)

  • Recombinant

Applications

  • WB

  • ICC

REACTIVITY

  • Human

Western blot analysis of Endo G on mouse heart tissue lysate using anti-Endo G antibody at 1/500 dilution.
  • Western blot analysis of Endo G on mouse heart tissue lysate using anti-Endo G antibody at 1/500 dilution.
  • ICC staining Endo G in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Endo G in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Endo G in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised
Western blot analysis of Endo G on mouse heart tissue lysate using anti-Endo G antibody at 1/500 dilution.

Applications

  • WB

  • ICC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Endo G Monoclonal Antibody (ET7106-77)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

MCF-7, PC-3M, SH-SY5Y, mouse heart tissue lysate.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU31-30

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

36 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Endo G

SYNONYMS

EndoG antibody; Endonuclease G antibody; Endonuclease G mitochondrial antibody; EndonucleaseG antibody; FLJ27463 antibody; Mitochondrial endonuclease G antibody; NUCG_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the DNA/RNA non-specific endonuclease family.

SUBCELLULAR LOCATION

Mitochondrion. Nucleus.

FUNCTION

Endonuclease G (ENDOG), a nuclear encoded protein, localizes to the mitochondria. This sugar-nonspecific nuclease, responsible for major mitochondrial nuclease activity, preferentially cleaves single-stranded DNA(ssDNA). Synthesized as a propeptide with an amino-terminal presequence that targets the nuclease to mitochondria, ENDOG translocates to neuclei on apoptotic stimulation and act as a nuclease without sequence specificity. Both exonucleases and DNase I stimulate the ability of ENDOG to generate double-stranded DNA cleavage products at physiological ionic strengths, suggesting that these activities work in concert with ENDOG in apoptotic cells to ensure efficient DNA breakdown. In addition to deoxyribonuclease activities, ENDOG also has ribonuclease (RNase) and RNase H activities. ENDOG is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. ENDOG exists in the mitochondrial intermembrane space, but not in the matrix where mtDNA replication occurs. This enzyme provides an important nicking function for mitochondrial DNA specifically cleaving DNA at GC tracts. Human ENDOG maps to chromosome 9q34.11.