PRODUCT CODE: ET1608-19

Recombinant ELK1 Monoclonal Antibody (ET1608-19)

  • Recombinant

Applications

  • WB

  • ICC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of ELK1 on human lung lysates using anti-ELK1 antibody at 1/1,000 dilution.
  • Western blot analysis of ELK1 on human lung lysates using anti-ELK1 antibody at 1/1,000 dilution.
  • ICC staining ELK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of ELK1 on human lung lysates using anti-ELK1 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant ELK1 Monoclonal Antibody (ET1608-19)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, human lung tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SU32-05

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

62 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

ELK1

SYNONYMS

ELK 1 antibody; Elk1 antibody; ELK1 member of ETS oncogene family antibody; ELK1 protein antibody; ELK1, ETS transcription factor antibody; ELK1_HUMAN antibody; ELK2 member of ETS oncogene family antibody; ETS domain containing protein Elk 1 antibody; ETS domain containing protein Elk1 antibody; ETS domain protein Elk1 antibody; ETS domain-containing protein Elk-1 antibody; ETS like gene 1 antibody; Member of ETS oncogene family antibody; Oncogene Elk1 antibody; Tyrosine kinase (ELK1) oncogene antibody

SEQUENCE SIMILARITIES

Belongs to the ETS family.

TISSUE SPECIFICITY

Lung and testis.

POST-TRANSLATIONAL MODIFICATION

Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.; On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Also phosphorylated on Ser-383 by MAPK8 and/or MAKP9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CAMK4, MAPK11, MAPK12 and MAPK14. Upon bFGF stimulus, phosphorylated by PAK1 (By similarity).

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1–3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF. Members of the Ets gene family exhibit varied patterns of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. Several of these proteins have been shown to recognize similar motifs in DNA that share a centrally located 5'-GGAA-3' element.