Positive control:
Lane 1: 293
Lane 2: A431
Lane 3: Jurkat
Applications
-
WB
-
IP
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant Eg5 Monoclonal Antibody (ET1706-03)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
293, A431, Jurkat.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JU83-16
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
119 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:2,000
-
IP
-
1:10-1:50
TARGET
UNIPROT #
PROTEIN NAME
Eg5
SYNONYMS
EG5 antibody; HKSP antibody; KIF11 antibody; KIF11_HUMAN antibody; Kinesin family member 11 antibody; Kinesin like protein 1 antibody; Kinesin-like protein 1 antibody; Kinesin-like protein KIF11 antibody; Kinesin-like spindle protein HKSP antibody; Kinesin-related motor protein Eg5 antibody; KNSL1 antibody; MCLMR antibody; Thyroid receptor-interacting protein 5 antibody; TR-interacting protein 5 antibody; TRIP-5 antibody; TRIP5 antibody
SEQUENCE SIMILARITIES
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. BimC subfamily.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase).; A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.
SUBCELLULAR LOCATION
Cytoplasm
FUNCTION
Eukaryotes contain a superfamily of microtubule-based motor proteins comprising kinesin and a number of related proteins that are thought to participate in various forms of intracellular motility, including cell division and organelle transport. KIF11(also known as kinesin family member 11, Eg5 or TRIP5) is a slow, plus-end-directed microtubule-based motor of the BimC kinesin family that is essential for bipolar spindle formation during eukaryotic cell division. When the expression of KIF11 is blocked, centrosome migration halts and cells are arrested in mitosis with monoastral microtubule arrays. KIF11 is phosphorylated on serine during S phase and on both serine and Thr 927 during mitosis, which regulates the association of Eg5 with the spindle apparatus (probably during early prophase). KIF11 is also known to be a member of the thyroid receptor interacting protein (Trip) family, and interacts with the thyroid hormone receptor only in the presence of thyroid hormone.