PRODUCT CODE: ET1706-03

Recombinant Eg5 Monoclonal Antibody (ET1706-03)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Human

Western blot analysis of Eg5 on different cell lysates using anti-Eg5 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: 293<br />
 Lane 2: A431<br />
 Lane 3: Jurkat<br />
  • Western blot analysis of Eg5 on different cell lysates using anti-Eg5 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: 293<br />
 Lane 2: A431<br />
 Lane 3: Jurkat<br />
Western blot analysis of Eg5 on different cell lysates using anti-Eg5 antibody at 1/500 dilution.
Positive control:
Lane 1: 293
Lane 2: A431
Lane 3: Jurkat

Applications

  • WB

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Eg5 Monoclonal Antibody (ET1706-03)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293, A431, Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU83-16

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

119 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

Kinesin-like protein KIF11

GENE NAME

KIF11

SYNONYMS

TR-interacting protein 5, TRIP-5, KIF11, EG5, KNSL1, TRIP5

SEQUENCE SIMILARITIES

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. BimC subfamily.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase).; A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.

SUBCELLULAR LOCATION

Cytoplasm. Cytoplasm, cytoskeleton, spindle pole.

FUNCTION

Motor protein required for establishing a bipolar spindle during mitosis. Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface.