PRODUCT CODE: ET1706-03

Recombinant Eg5 Monoclonal Antibody (ET1706-03)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Human

Western blot analysis of Eg5 on different cell lysates using anti-Eg5 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: 293<br />
  Lane 2: A431<br />
  Lane 3: Jurkat
  • Western blot analysis of Eg5 on different cell lysates using anti-Eg5 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: 293<br />
  Lane 2: A431<br />
  Lane 3: Jurkat
Western blot analysis of Eg5 on different cell lysates using anti-Eg5 antibody at 1/500 dilution.
Positive control:
Lane 1: 293
Lane 2: A431
Lane 3: Jurkat

Applications

  • WB

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Eg5 Monoclonal Antibody (ET1706-03)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293, A431, Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU83-16

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

119 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

Eg5

SYNONYMS

EG5 antibody; HKSP antibody; KIF11 antibody; KIF11_HUMAN antibody; Kinesin family member 11 antibody; Kinesin like protein 1 antibody; Kinesin-like protein 1 antibody; Kinesin-like protein KIF11 antibody; Kinesin-like spindle protein HKSP antibody; Kinesin-related motor protein Eg5 antibody; KNSL1 antibody; MCLMR antibody; Thyroid receptor-interacting protein 5 antibody; TR-interacting protein 5 antibody; TRIP-5 antibody; TRIP5 antibody

SEQUENCE SIMILARITIES

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. BimC subfamily.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase).; A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.

SUBCELLULAR LOCATION

Cytoplasm

FUNCTION

Eukaryotes contain a superfamily of microtubule-based motor proteins comprising kinesin and a number of related proteins that are thought to participate in various forms of intracellular motility, including cell division and organelle transport. KIF11(also known as kinesin family member 11, Eg5 or TRIP5) is a slow, plus-end-directed microtubule-based motor of the BimC kinesin family that is essential for bipolar spindle formation during eukaryotic cell division. When the expression of KIF11 is blocked, centrosome migration halts and cells are arrested in mitosis with monoastral microtubule arrays. KIF11 is phosphorylated on serine during S phase and on both serine and Thr 927 during mitosis, which regulates the association of Eg5 with the spindle apparatus (probably during early prophase). KIF11 is also known to be a member of the thyroid receptor interacting protein (Trip) family, and interacts with the thyroid hormone receptor only in the presence of thyroid hormone.