PRODUCT CODE: ET1701-96

Recombinant EBP50 Monoclonal Antibody (ET1701-96)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Rat

Western blot analysis of EBP50 on MCF-7 cells lysates using anti-EBP50 antibody at 1/1,000 dilution.
  • Western blot analysis of EBP50 on MCF-7 cells lysates using anti-EBP50 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-EBP50 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-EBP50 antibody. Counter stained with hematoxylin.
Western blot analysis of EBP50 on MCF-7 cells lysates using anti-EBP50 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant EBP50 Monoclonal Antibody (ET1701-96)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

MCF-7, rat kidney tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ0946

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Na(+)/H(+) exchange regulatory cofactor NHE-RF1

GENE NAME

SLC9A3R1

SYNONYMS

NHERF-1, EBP50, SLC9A3R1

TISSUE SPECIFICITY

Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on serine residues.

SUBCELLULAR LOCATION

Cytoplasm. Apical cell membrane. Endomembrane system; Peripheral membrane protein. Cell projection, filopodium. Cell projection, ruffle. Cell projection, microvillus. Note=Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner. Colocalizes with CFTR at the midpiece of sperm tail (By similarity). Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T-cells.

FUNCTION

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Involved in the regulation of phosphate reabsorption in the renal proximal tubules. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa.