PRODUCT CODE: ET7106-99

Recombinant EB3 Monoclonal Antibody (ET7106-99)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of EB3 on different lysates using anti-EB3 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse brain tissue<br />
 Lane 2: K562<br />
  • Western blot analysis of EB3 on different lysates using anti-EB3 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse brain tissue<br />
 Lane 2: K562<br />
  • ICC staining EB3 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining EB3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining EB3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-EB3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-EB3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-EB3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-EB3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY-5Y cells with EB3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of EB3 on different lysates using anti-EB3 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse brain tissue
Lane 2: K562

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant EB3 Monoclonal Antibody (ET7106-99)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, PC-3M, SH-SY-5Y, rat cerebellum tissue, human lung cancer tissue, mouse brain tissue, mouse fallopian tubes tissue, K562.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU38-30

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

32 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Microtubule-associated protein RP/EB family member 3

GENE NAME

MAPRE3

SYNONYMS

EBF3, EB3, MAPRE3

SEQUENCE SIMILARITIES

Belongs to the MAPRE family.

TISSUE SPECIFICITY

Predominantly expressed in brain and muscle.

SUBCELLULAR LOCATION

Cytoplasm, cytoskeleton. Note=Associated with the microtubule network. Detected at the plus end of microtubules.

FUNCTION

Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules. May play a role in cell migration (By similarity).