PRODUCT CODE: ET1611-88

Recombinant E2F2 Monoclonal Antibody (ET1611-88)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of E2F2 on different lysates using anti-E2F2 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: K562 <br />
Lane 2: 293T <br />
Lane 3: HepG2
  • Western blot analysis of E2F2 on different lysates using anti-E2F2 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: K562 <br />
Lane 2: 293T <br />
Lane 3: HepG2
  • Flow cytometric analysis of K562 cells with E2F2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of E2F2 on different lysates using anti-E2F2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: K562
Lane 2: 293T
Lane 3: HepG2

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant E2F2 Monoclonal Antibody (ET1611-88)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

K562, 293T, HepG2.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN201-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

48 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Transcription factor E2F2

GENE NAME

E2F2

SYNONYMS

E2F-2, E2F2

SEQUENCE SIMILARITIES

Belongs to the E2F/DP family.

TISSUE SPECIFICITY

Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.