PRODUCT CODE: ET1705-2

Recombinant Dynamin 2 Monoclonal Antibody (ET1705-2)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of Dynamin 2 on different cell lysate using anti-Dynamin 2 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: PC-12<br />
 Lane 2: Mouse thymus<br />
 Lane 3: Hela<br />
  • Western blot analysis of Dynamin 2 on different cell lysate using anti-Dynamin 2 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: PC-12<br />
 Lane 2: Mouse thymus<br />
 Lane 3: Hela<br />
  • ICC staining Dynamin 2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Dynamin 2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Dynamin 2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Dynamin 2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testes tissue using anti-Dynamin 2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Dynamin 2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-Dynamin 2 antibody. Counter stained with hematoxylin.
  •  Flow cytometric analysis of Hela cells with Dynamin 2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of Dynamin 2 on different cell lysate using anti-Dynamin 2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: PC-12
Lane 2: Mouse thymus
Lane 3: Hela

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Dynamin 2 Monoclonal Antibody (ET1705-2)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC-12, mouse thymus tissue, Hela, 293T, human kidney tissue, human fetal skeletal muscle tissue, mouse testes tissue, mouse colon tissue, mouse fallopian tube tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM61-38

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

98 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Dynamin-2

GENE NAME

DNM2

SYNONYMS

DNM2, DYN2

SEQUENCE SIMILARITIES

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.

TISSUE SPECIFICITY

Ubiquitously expressed.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-764 by CDK1 is greatly increased upon mitotic entry. It regulates cytokinesis downstream of calcineurin, and does not affect clathrin-mediated endocytosis. Dephosphorylated by calcineurin/PP2 (By similarity). Phosphorylated on tyrosine residues after activation of SRC (By similarity).

SUBCELLULAR LOCATION

Cytoplasm. Cytoplasm, cytoskeleton. Cell junction. Membrane, clathrin-coated pit. Cell junction, synapse, postsynaptic density. Cell junction, synapse. Midbody. Cell projection, phagocytic cup. Cytoplasmic vesicle, phagosome membrane; Peripheral membrane protein. Note=Colocalizes with CTTN at the basis of filopodia in hippocampus neuron growth zones (By similarity). Microtubule-associated. Also found in the postsynaptic density of neuronal cells. Co-localizes with PIK3C3 and RAB5A to the nascent phagosome (By similarity).

FUNCTION

Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Plays an important role in vesicular trafficking processes, in particular endocytosis. Involved in cytokinesis. Regulates maturation of apoptotic cell corpse-containing phagosomes by recruiting PIK3C3 to the phagosome membrane (By similarity).