Positive control:
Lane 1: NIH/3T3 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: mouse spleen tissue lysate
Applications
-
WB
-
IHC-P
-
IP
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant DUSP1 Monoclonal Antibody (ET1701-82)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
NIH/3T3 cell lysate, Jurkat cell lysate, mouse spleen tissue lysate, human lung carcinoma tissue, mouse lung tissue, HepG2.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JJ0930
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
40 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000
-
FC
-
1:50-1:100
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
DUSP1
SYNONYMS
CL 100 antibody; CL100 antibody; Dual Specificity Phosphatase 1 antibody; Dual specificity protein phosphatase 1 antibody; Dual specificity protein phosphatase hVH1 antibody; DUS1_HUMAN antibody; DUSP 1 antibody; Dusp1 antibody; HVH1 antibody; MAP kinase phosphatase 1 antibody; Mitogen-activated protein kinase phosphatase 1 antibody; MKP-1 antibody; MKP1 antibody; Protein tyrosine phosphatase CL100 antibody; Protein-tyrosine phosphatase CL100 antibody; PTPN10 antibody; Serine/threonine specific protein phosphatase antibody; VH1 antibody
SEQUENCE SIMILARITIES
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
TISSUE SPECIFICITY
Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Ser-359 and Ser-364 by MAPK1/ERK2 and MAPK3/ERK1 reduces its rate of degradation.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
A key element in the pathway involved in the transduction of signals from activated protein-tyrosine kinase transmembrane receptors has been identified as the family of mitogen-activated protein kinases (MAP kinases). The most well known of these Ser/Thr kinases are ERK 1 and ERK 2. Mitogenic stimulation of cells triggers the activation of MAP kinases through phosphorylation of both tyrosyl (Y185) and threonyl (T183) residues. Phosphorylation of the T183 and Y185 ERK regulatory site is mediated by MAP kinase (MEK), which in turn is regulated by the proto-oncogene product Raf. Two highly related phosphatases, designated MKP-1 and MKP-2, exhibit 59% sequence identity at the amino acid level and oppose the action of MEK by downregulating the kinase activity of ERK 1 and ERK 2. MAP kinase phosphatase-1 and -2 proteins function by dephosphorylating ERK 1 and ERK 2 at their T-E-Y regulatory motif. An additional phosphatase encoded by the DUSP2 gene, designated PAC-1, also functions to downregulate ERK 1 and ERK 2 kinase activity. PAC-1 is a nuclear protein whose expression is strongly induced in response to mitogen.