PRODUCT CODE: ET1703-45

Recombinant DRD1 Monoclonal Antibody (ET1703-45)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of DRD1 on mouse kidney cells lysates using anti-DRD1 antibody at 1/500 dilution.
  • Western blot analysis of DRD1 on mouse kidney cells lysates using anti-DRD1 antibody at 1/500 dilution.
  • ICC staining DRD1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DRD1 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DRD1 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DRD1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DRD1 antibody. Counter stained with hematoxylin.
Western blot analysis of DRD1 on mouse kidney cells lysates using anti-DRD1 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant DRD1 Monoclonal Antibody (ET1703-45)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293T, N2A, SH-SY5Y, mouse kidney, mouse brain tissue, rat brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-93

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

75 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

D(1A) dopamine receptor

GENE NAME

DRD1

SYNONYMS

DRD1

SEQUENCE SIMILARITIES

Belongs to the G-protein coupled receptor 1 family.

TISSUE SPECIFICITY

Detected in caudate, nucleus accumbens and in the olfactory tubercle.

SUBCELLULAR LOCATION

Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell projection, dendrite. Cell projection, dendritic spine. Note=Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14.

FUNCTION

Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.