PRODUCT CODE: ET1703-45

Recombinant DRD1 Monoclonal Antibody (ET1703-45)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of DRD1 on mouse kidney cells lysates using anti-DRD1 antibody at 1/500 dilution.
  • Western blot analysis of DRD1 on mouse kidney cells lysates using anti-DRD1 antibody at 1/500 dilution.
  • ICC staining DRD1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DRD1 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DRD1 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DRD1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DRD1 antibody. Counter stained with hematoxylin.
Western blot analysis of DRD1 on mouse kidney cells lysates using anti-DRD1 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant DRD1 Monoclonal Antibody (ET1703-45)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293T, N2A, SH-SY5Y, mouse kidney, mouse brain tissue, rat brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-93

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

75 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

DRD1

SYNONYMS

D(1A) dopamine receptor antibody; D1A dopamine receptor antibody; DADR antibody; Dopamine D1 receptor antibody; dopamine receptor D1 antibody; DR D1 antibody; DR D1A antibody; DRD 1 antibody; DRD 1A antibody; DRD1 antibody; DRD1_HUMAN antibody; DRD1A antibody

SEQUENCE SIMILARITIES

Belongs to the G-protein coupled receptor 1 family.

TISSUE SPECIFICITY

Detected in caudate, nucleus accumbens and in the olfactory tubercle.

SUBCELLULAR LOCATION

Cell membrane. Endoplasmic reticulum membrane.

FUNCTION

The members of the G protein coupled receptor family are distinguished by their slow transmitting response to ligand binding. These transmembrane proteins include the adrenergic, serotonin and dopamine receptors. The effect of the signaling molecule can be excitatory or inhibitory depending on the type of receptor to which it binds. β-adrenergic receptor binds to adrenaline activates adenylyl cyclase, while α2-adrenergic receptor binds to adrenaline inhibits adenylyl cyclase. The dopamine receptors are divided into two classes, D1 and D2, which differ in their functional characteristics in that D1 receptors stimulate adenylyl cyclase while D2 receptors inhibit adenylyl cyclase activity. Five different subtypes of dopamine receptor have been described to date. D1DR and D5DR belong to the D1 subclass, while D2DR, D3DR and D4DR belong to the D2 subclass.