Lane 1: Hela cell lysate
Lane 2: human brain tissue lysate
Lane 3: human heart tissue lysate
Recombinant Rabbit monoclonal primary
Recombinant Dnmt3a Monoclonal Antibody (ET1609-31)
NIH/3T3, Hela, HepG2, human brain tissue, human heart tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A purified.
Dnmt3a, DNA MTase HsaIIIA, M.HsaIIIA, DNMT3A
Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.
Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.
Nucleus. Cytoplasm. Note=Accumulates in the major satellite repeats at pericentric heterochromatin.
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.