PRODUCT CODE: ET1705-12

Recombinant DNA Polymerase beta Monoclonal Antibody (ET1705-12)

  • Zebrafish
  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

Western blot analysis of DNA Polymerase beta on different cell lysate using anti-DNA Polymerase beta antibody at 1/1,000 dilution.<br />
  Positive control:<br />
  Lane 1: PC-12 <br />
  Lane 2: NIH-3T3 <br />
  Lane 3: A431
  • Western blot analysis of DNA Polymerase beta on different cell lysate using anti-DNA Polymerase beta antibody at 1/1,000 dilution.<br />
  Positive control:<br />
  Lane 1: PC-12 <br />
  Lane 2: NIH-3T3 <br />
  Lane 3: A431
  • Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-DNA Polymerase beta antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-DNA Polymerase beta antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-DNA Polymerase beta antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-DNA Polymerase beta antibody. Counter stained with hematoxylin.
  • Western blot analysis of DNA Polymerase beta on Zebrafish tissue lysates using anti-DNA Polymerase beta antibody at 1/200 dilution.
Western blot analysis of DNA Polymerase beta on different cell lysate using anti-DNA Polymerase beta antibody at 1/1,000 dilution.
Positive control:
Lane 1: PC-12
Lane 2: NIH-3T3
Lane 3: A431

Applications

  • WB

  • IHC-P

  • IP

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant DNA Polymerase beta Monoclonal Antibody (ET1705-12)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC-12, NIH-3T3, A431, Zebrafish tissue lysates, rat lung tissue, human breast cancer tissue, human stomach cancer tissue, human uterus tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM93-12

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

38 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:200

  • ICC

  • 1:20-1:50 IP

TARGET

UNIPROT #

PROTEIN NAME

DNA Polymerase beta

SYNONYMS

DNA directed DNA polymerase beta antibody; DNA pol beta antibody; DNA polymerase beta antibody; DNA polymerase beta subunit antibody; DPOLB_HUMAN antibody; MGC125976 antibody; Pol B antibody; Pol beta antibody; POLB antibody; Polymerase (DNA directed) beta antibody

SEQUENCE SIMILARITIES

Belongs to the DNA polymerase type-X family.

POST-TRANSLATIONAL MODIFICATION

Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.; Ubiquitinated at Lys-41, Lys-61 and Lys-81: monoubiquitinated by HUWE1/ARF-BP1. Monoubiquitinated protein is then the target of STUB1/CHIP, which catalyzes polyubiquitination from monoubiquitin, leading to degradation by the proteasome. USP47 mediates the deubiquitination of monoubiquitinated protein, preventing polyubiquitination by STUB1/CHIP and its subsequent degradation.

SUBCELLULAR LOCATION

Nucleus, Cytoplasm.

FUNCTION

DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches. These exonucleases include the family of DNA polymerases. DNA pol α, β, δ, and e are involved in DNA replication and repair. DNA pol δ and DNA pol e are multisubunit enzymes, with DNA pol δ consisting of two subunits p125, which interacts with the sliding DNA clamp protein PCNA, and p50. The nuclear-encoded DNA pol γ is the only DNA polymerase required for the replication of the mitochondrial DNA. DNA pol ζ is ubiquitously expressed in various tissues and mediates the cellular mechanism of damage-induced mutagenesis. DNA pol œ is a DNA polymerase-helicase that binds ATP and is involved in the repair of interstrand crosslinks.