Positive control:
Lane 1: HepG2 Lane 2: Hela Lane 3: Jurkat
Applications
-
WB
-
IHC-P
-
ICC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant DNA Ligase IV Monoclonal Antibody (ET1705-77)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
HepG2, Hela, Jurkat, human colon cancer tissue, human placenta tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM64-32
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
104 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:2,000
-
IHC-P
-
1:50-1:200
-
ICC
-
1:10-1:50
TARGET
UNIPROT #
PROTEIN NAME
DNA Ligase IV
SYNONYMS
DNA ligase IV ATP dependent antibody; DNA joinase antibody; DNA ligase 4 antibody; DNA ligase IV antibody; DNA repair enzyme antibody; DNLI4_HUMAN antibody; LIG 4 antibody; LIG4 antibody; LIG4S antibody; Ligase IV antibody; Ligase IV DNA ATP dependent antibody; Polydeoxyribonucleotide synthase [ATP] 4 antibody; Polydeoxyribonucleotide synthase 4 antibody; Polydeoxyribonucleotide synthase antibody; Polynucleotide ligase antibody; Sealase antibody
SEQUENCE SIMILARITIES
Belongs to the ATP-dependent DNA ligase family.
TISSUE SPECIFICITY
Testis, thymus, prostate and heart.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
The X-ray repair cross-complementing protein XRCC4 and DNA Ligase IV are essential for repairing double-strand breaks in DNA. These stories form a critical complex composed of two molecules of each protein that preferentially bind DNA with nicks or broken ends. As an obligatory accessory molecule, XRCC4 binds to DNA Ligase IV and enhances its joining activity. The XRCC4 / DNA Ligase IV complex is also involved in V (D) J recombination. V (D) (in) a pair of high incidence of apoptosis in the development nervous system and a block in B and T cell maturation.