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PRODUCT CODE: ET1705-77

Recombinant DNA Ligase IV Monoclonal Antibody (ET1705-77)

  • Recombinant

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

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Western blot analysis of DNA Ligase IV on Hela cell using anti-DNA Ligase IV antibody at 1/1,000 dilution.<br /> Positive control:<br /> Lane 1: HepG2 Lane 2: Hela Lane 3: Jurkat
  • Western blot analysis of DNA Ligase IV on Hela cell using anti-DNA Ligase IV antibody at 1/1,000 dilution.<br /> Positive control:<br /> Lane 1: HepG2 Lane 2: Hela Lane 3: Jurkat
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-DNA Ligase IV antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DNA Ligase IV antibody. Counter stained with hematoxylin.
Western blot analysis of DNA Ligase IV on Hela cell using anti-DNA Ligase IV antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2 Lane 2: Hela Lane 3: Jurkat

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant DNA Ligase IV Monoclonal Antibody (ET1705-77)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HepG2, Hela, Jurkat, human colon cancer tissue, human placenta tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM64-32

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

104 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • ICC

  • 1:10-1:50

TARGET

UNIPROT #

P49917

PROTEIN NAME

DNA Ligase IV

SYNONYMS

DNA ligase IV ATP dependent antibody; DNA joinase antibody; DNA ligase 4 antibody; DNA ligase IV antibody; DNA repair enzyme antibody; DNLI4_HUMAN antibody; LIG 4 antibody; LIG4 antibody; LIG4S antibody; Ligase IV antibody; Ligase IV DNA ATP dependent antibody; Polydeoxyribonucleotide synthase [ATP] 4 antibody; Polydeoxyribonucleotide synthase 4 antibody; Polydeoxyribonucleotide synthase antibody; Polynucleotide ligase antibody; Sealase antibody

SEQUENCE SIMILARITIES

Belongs to the ATP-dependent DNA ligase family.

TISSUE SPECIFICITY

Testis, thymus, prostate and heart.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The X-ray repair cross-complementing protein XRCC4 and DNA Ligase IV are essential for repairing double-strand breaks in DNA. These stories form a critical complex composed of two molecules of each protein that preferentially bind DNA with nicks or broken ends. As an obligatory accessory molecule, XRCC4 binds to DNA Ligase IV and enhances its joining activity. The XRCC4 / DNA Ligase IV complex is also involved in V (D) J recombination. V (D) (in) a pair of high incidence of apoptosis in the development nervous system and a block in B and T cell maturation.