PRODUCT CODE: ET1705-77

Recombinant DNA Ligase IV Monoclonal Antibody (ET1705-77)

  • Recombinant

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

Western blot analysis of DNA Ligase IV on Hela cell using anti-DNA Ligase IV antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: HepG2 Lane 2: Hela Lane 3: Jurkat
  • Western blot analysis of DNA Ligase IV on Hela cell using anti-DNA Ligase IV antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: HepG2 Lane 2: Hela Lane 3: Jurkat
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-DNA Ligase IV antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DNA Ligase IV antibody. Counter stained with hematoxylin.
Western blot analysis of DNA Ligase IV on Hela cell using anti-DNA Ligase IV antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2 Lane 2: Hela Lane 3: Jurkat

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant DNA Ligase IV Monoclonal Antibody (ET1705-77)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HepG2, Hela, Jurkat, human colon cancer tissue, human placenta tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM64-32

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

104 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • ICC

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

DNA ligase 4

GENE NAME

LIG4

SYNONYMS

LIG4

SEQUENCE SIMILARITIES

Belongs to the ATP-dependent DNA ligase family.

TISSUE SPECIFICITY

Testis, thymus, prostate and heart.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.