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PRODUCT CODE: ET1610-74

Recombinant DIABLO Monoclonal Antibody (ET1610-74)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of DIABLO on different lysates using anti-DIABLO antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: Jurkat <br /> Lane 2: Hela <br /> Lane 3: MCF-7
  • Western blot analysis of DIABLO on different lysates using anti-DIABLO antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: Jurkat <br /> Lane 2: Hela <br /> Lane 3: MCF-7
  • ICC staining DIABLO in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DIABLO in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DIABLO in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DIABLO in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-DIABLO antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse overy tissue using anti-DIABLO antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-DIABLO antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with DIABLO antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of DIABLO on different lysates using anti-DIABLO antibody at 1/1,000 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: Hela
Lane 3: MCF-7

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant DIABLO Monoclonal Antibody (ET1610-74)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat, MCF-7, Hela, B16-F1, LO2, N2A, mouse overy tissue, mouse skin tissue, human spleen tissue, mouse liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC67-06

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

21 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

Q9NR28

PROTEIN NAME

DIABLO

SYNONYMS

0610041G12Rik antibody; DBLOH_HUMAN antibody; DBOH antibody; DFNA64 antibody; diablo antibody; Diablo homolog (Drosophila) antibody; Diablo homolog antibody; Diablo homolog mitochondrial antibody; Diablo IAP binding mitochondrial protein antibody; Diablo like protein antibody; DIABLO S antibody; Direct IAP binding protein with low pI antibody; Direct IAP-binding protein with low pI antibody; FLJ10537 antibody; FLJ25049 antibody; mitochondrial antibody; Mitochondrial Smac protein antibody; Second mitochondria derived activator of caspase antibody; Second mitochondria-derived activator of caspase antibody; SMAC 3 antibody; Smac antibody; Smac protein antibody; SMAC3 antibody

TISSUE SPECIFICITY

Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated by BIRC7/livin.

SUBCELLULAR LOCATION

Mitochondrion.

FUNCTION

The activation of caspases is a key regulatory step in apoptosis. Once cytochrome c is released from the mitochondria into the cytosol, it binds Apaf-1 to form an oligomeric cytochrome c/Apaf-1 complex, which induces caspase activation. Inhibitors of Apoptosis Proteins (IAPs), are a family of proteins that regulate the cytochrome c/Apaf-1 caspase activating pathway. Like cytochrome c, Smac (for second mitochondria-derived activator of caspase, also designated DIABLO in mouse for direct IAP binding protein with low PI) promotes caspase activation in the cytochrome c/Apaf-1/caspase-9 pathway by binding IAPs and preventing them from inhibiting caspases. In healthy cells, Smac is a mitochondrial protein, but when cells undergo apoptosis, Smac is released into the cytosol.