PRODUCT CODE: ET1703-88

Recombinant delta Sarcoglycan Monoclonal Antibody (ET1703-88)

  • Recombinant

Applications

  • WB

  • IP

  • IHC-P

REACTIVITY

  • Human

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Western blot analysis of delta Sarcoglycan on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-88, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of delta Sarcoglycan on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-88, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-delta Sarcoglycan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-88, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of delta Sarcoglycan on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-88, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IP

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant delta Sarcoglycan Monoclonal Antibody (ET1703-88)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human lung tissue lysate, human skeletal muscle tissue lysate, human stomach carcinoma tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM61-10

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

35 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

delta Sarcoglycan

SYNONYMS

35 kDa dystrophin associated glycoprotein antibody; 35 kDa dystrophin-associated glycoprotein antibody; 35DAG antibody; CMD1L antibody; DAGD antibody; Delta-sarcoglycan antibody; Delta-SG antibody; Dystrophin associated glycoprotein delta sarcoglycan antibody; LGMD2F antibody; MGC22567 antibody; Placental delta sarcoglycan antibody; Sarcoglycan delta (35 kDa dystrophin associated glycoprotein) antibody; SG delta antibody; SGCD antibody; SGCD_HUMAN antibody; SGCDP antibody; SGD antibody

SEQUENCE SIMILARITIES

Belongs to the sarcoglycan beta/delta/gamma/zeta family.

TISSUE SPECIFICITY

Most strongly expressed in skeletal and cardiac muscle. Also detected in smooth muscle. Weak expression in brain and lung.

POST-TRANSLATIONAL MODIFICATION

Glycosylated.; Disulfide bonds are present.

SUBCELLULAR LOCATION

Cytoskeleton, sarcolemma.

FUNCTION

Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix. Defects in SGCD are the cause of limb-girdle muscular dystrophy type 2F (LGMD2F) [MIM:601287]. LGMD2F is an autosomal recessive disorder. Defects in SGCD are the cause of cardiomyopathy dilated type 1L (CMD1L) [MIM:606685]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.