PRODUCT CODE: ET1611-92

Recombinant Delta Opioid Receptor Monoclonal Antibody (ET1611-92)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of OPRD1 on different lysates using anti-OPRD1 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: HUVEC <br />
Lane 2: Rat brain
  • Western blot analysis of OPRD1 on different lysates using anti-OPRD1 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: HUVEC <br />
Lane 2: Rat brain
  • Flow cytometric analysis of SH-SY-5Y cells with OPRD1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of OPRD1 on different lysates using anti-OPRD1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HUVEC
Lane 2: Rat brain

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Delta Opioid Receptor Monoclonal Antibody (ET1611-92)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HUVEC, rat brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN20-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

40 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Delta-type opioid receptor

GENE NAME

OPRD1

SYNONYMS

D-OR-1, DOR-1, OPRD1

SEQUENCE SIMILARITIES

Belongs to the G-protein coupled receptor 1 family.

TISSUE SPECIFICITY

Detected in oocytes (at protein level). Detected in brain cortex, hypothalamus, hippocampus and olfactory bulb. Detected in oocytes.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated.; Ubiquitinated. A basal ubiquitination seems not to be related to degradation. Ubiquitination is increased upon formation of OPRM1:OPRD1 oligomers leading to proteasomal degradation; the ubiquitination is diminished by RTP4.

SUBCELLULAR LOCATION

Cell membrane; Multi-pass membrane protein.

FUNCTION

G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine.