PRODUCT CODE: ET7107-06

Recombinant DDX6 Monoclonal Antibody (ET7107-06)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of DDX6 on different lysates using anti-DDX6 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Human colon <br />
 Lane 2: SH-SY-5Y<br />
 Lane 3: SiHa <br />
 Lane 4: K562<br />
  • Western blot analysis of DDX6 on different lysates using anti-DDX6 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Human colon <br />
 Lane 2: SH-SY-5Y<br />
 Lane 3: SiHa <br />
 Lane 4: K562<br />
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-DDX6 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DDX6 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-DDX6 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DDX6 antibody. Counter stained with hematoxylin.
Western blot analysis of DDX6 on different lysates using anti-DDX6 antibody at 1/500 dilution.
Positive control:
Lane 1: Human colon
Lane 2: SH-SY-5Y
Lane 3: SiHa
Lane 4: K562

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant DDX6 Monoclonal Antibody (ET7107-06)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat brain tissue, human tonsil tissue, human kidney tissue, mouse small intestine tissue, human colon tissue, SH-SY-5Y, K562, SiHa.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB41-32

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

54 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Probable ATP-dependent RNA helicase DDX6

GENE NAME

DDX6

SYNONYMS

DDX6, HLR2, RCK

SEQUENCE SIMILARITIES

Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily.

TISSUE SPECIFICITY

Abundantly expressed in most tissues.

POST-TRANSLATIONAL MODIFICATION

Sumoylated.

SUBCELLULAR LOCATION

Cytoplasm, P-body. Cytoplasm. Nucleus. Note=Upon cellular stress, relocalizes to stress granules.

FUNCTION

Essential for the formation of P-bodies, cytosolic membrane-less ribonucleoprotein granules involved in RNA metabolism through the coordinated storage of mRNAs encoding regulatory functions. In the process of mRNA degradation, plays a role in mRNA decapping. Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts.