PRODUCT CODE: ET1705-32

Recombinant DDX5 Monoclonal Antibody (ET1705-32)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

 Western blot analysis of DDX5 on different cell lysate using anti-DDX5 antibody at 1/1,000 dilution.<br />
Positive control:<br />
 Lane 1: PC-12 <br />
 Lane 2: MEF <br />
 Lane 3: SH-SY5Y <br />
 Lane 4: NIH-3T3 <br />
 Lane 5: Mouse brain <br />
  •  Western blot analysis of DDX5 on different cell lysate using anti-DDX5 antibody at 1/1,000 dilution.<br />
Positive control:<br />
 Lane 1: PC-12 <br />
 Lane 2: MEF <br />
 Lane 3: SH-SY5Y <br />
 Lane 4: NIH-3T3 <br />
 Lane 5: Mouse brain <br />
  • ICC staining DDX5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DDX5 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DDX5 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DDX5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DDX5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DDX5 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY5Y cells with DDX5 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of DDX5 on different cell lysate using anti-DDX5 antibody at 1/1,000 dilution.
Positive control:
Lane 1: PC-12
Lane 2: MEF
Lane 3: SH-SY5Y
Lane 4: NIH-3T3
Lane 5: Mouse brain

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant DDX5 Monoclonal Antibody (ET1705-32)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC-12 cell lysate, MEF cell lysate, SH-SY5Y, NIH-3T3 cell lysate, mouse brain tissue lysate, Hela, SW480, rat brain tissue, human placenta tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM52-30

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

69/60 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Probable ATP-dependent RNA helicase DDX5

GENE NAME

DDX5

SYNONYMS

G17P1, HELR, HLR1

SEQUENCE SIMILARITIES

Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.

POST-TRANSLATIONAL MODIFICATION

Arg-502 is dimethylated, probably to asymmetric dimethylarginine.; Sumoylated; sumoylation, promoted by PIAS1, promotes interaction with HDAC1 and transcriptional repression activity. Sumoylation also significantly increases stability, and reduces polyubiquitination.; Polyubiquitinated, leading to proteasomal degradation.; Weakly phosphorylated in the G1/S phase of the cell cycle and much more at G2/M, especially at Thr and Tyr residues.

SUBCELLULAR LOCATION

Nucleus. Nucleus, nucleolus. Cytoplasm. Note=During the G0 phase, predominantly located in the nucleus. Cytoplasmic levels increase during the G1/S phase. During the M phase, located at the vicinity of the condensed chromosomes. At G1, localizes in the cytoplasm.

FUNCTION

Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specific manner; the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.