PRODUCT CODE: ET1706-22

Recombinant DDB1 Monoclonal Antibody (ET1706-22)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of DDB1 on different lysates using anti-DDB1 antibody at 1/500 dilution.<br />
Positive control:<br />
 Lane 1: HepG2<br />
 Lane 2: NIH-3T3<br />
 Lane 3: MCF-7<br />
 Lane 4: Rat kidney tissue<br />
  • Western blot analysis of DDB1 on different lysates using anti-DDB1 antibody at 1/500 dilution.<br />
Positive control:<br />
 Lane 1: HepG2<br />
 Lane 2: NIH-3T3<br />
 Lane 3: MCF-7<br />
 Lane 4: Rat kidney tissue<br />
  • ICC staining DDB1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DDB1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DDB1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat esophagus tissue using anti-DDB1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-DDB1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DDB1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DDB1 antibody. Counter stained with hematoxylin.
Western blot analysis of DDB1 on different lysates using anti-DDB1 antibody at 1/500 dilution.
Positive control:
Lane 1: HepG2
Lane 2: NIH-3T3
Lane 3: MCF-7
Lane 4: Rat kidney tissue

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant DDB1 Monoclonal Antibody (ET1706-22)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HepG2, NIH-3T3, MCF-7, rat kidney tissue lysate, Hela, HUVEC, SH-SY5Y, rat esophagus tissue, human liver cancer tissue, human kidney tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU32-35

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

127 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

DNA damage-binding protein 1

GENE NAME

DDB1

SYNONYMS

DDBa, XAP-1, UV-DDB 1, XPE-BF, XPCe, DDB1, XAP1

SEQUENCE SIMILARITIES

Belongs to the DDB1 family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by ABL1.; Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis.; Acetylated, promoting interaction with CUL4 (CUL4A or CUL4B) and subsequent formation of DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes. Deacetylation by SIRT7 impairs the interaction with CUL4 (CUL4A or CUL4B) and formation of DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus. Note=Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage.

FUNCTION

Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively. Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1. DDB1-mediated CRY1 degradation promotes FOXO1 protein stability and FOXO1-mediated gluconeogenesis in the liver (By similarity).