PRODUCT CODE: ET1706-22

Recombinant DDB1 Monoclonal Antibody (ET1706-22)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of DDB1 on different lysates using anti-DDB1 antibody at 1/500 dilution.<br />
 Positive control:<br />
  Lane 1: HepG2<br />
  Lane 2: NIH-3T3<br />
  Lane 3: MCF-7<br />
  Lane 4: Rat kidney tissue
  • Western blot analysis of DDB1 on different lysates using anti-DDB1 antibody at 1/500 dilution.<br />
 Positive control:<br />
  Lane 1: HepG2<br />
  Lane 2: NIH-3T3<br />
  Lane 3: MCF-7<br />
  Lane 4: Rat kidney tissue
  • ICC staining DDB1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DDB1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DDB1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat esophagus tissue using anti-DDB1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-DDB1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DDB1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DDB1 antibody. Counter stained with hematoxylin.
Western blot analysis of DDB1 on different lysates using anti-DDB1 antibody at 1/500 dilution.
Positive control:
Lane 1: HepG2
Lane 2: NIH-3T3
Lane 3: MCF-7
Lane 4: Rat kidney tissue

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant DDB1 Monoclonal Antibody (ET1706-22)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HepG2, NIH-3T3, MCF-7, rat kidney tissue lysate, Hela, HUVEC, SH-SY5Y, rat esophagus tissue, human liver cancer tissue, human kidney tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU32-35

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

127 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

DDB1

SYNONYMS

Damage specific DNA binding protein 1 antibody; Damage-specific DNA-binding protein 1 antibody; DDB 1 antibody; DDB p127 subunit antibody; Ddb1 antibody; DDB1_HUMAN antibody; DDBa antibody; DNA damage binding protein 1 antibody; DNA damage-binding protein 1 antibody; DNA damage-binding protein a antibody; HBV X-associated protein 1 antibody; UV damaged DNA binding factor antibody; UV damaged DNA binding protein 1 antibody; UV DDB 1 antibody; UV DDB1 antibody; UV-damaged DNA-binding factor antibody; UV-damaged DNA-binding protein 1 antibody; UV-DDB 1 antibody; X associated protein 1 antibody; XAP 1 antibody; XAP-1 antibody; XAP1 antibody; Xeroderma pigmentosum group E complementing protein antibody; Xeroderma pigmentosum group E-complementing protein antibody; XPCe antibody; XPE antibody; XPE BF antibody; XPE binding factor antibody; XPE-BF antibody; XPE-binding factor antibody

SEQUENCE SIMILARITIES

Belongs to the DDB1 family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by ABL1.; Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis.; Acetylated, promoting interaction with CUL4 (CUL4A or CUL4B) and subsequent formation of DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes. Deacetylation by SIRT7 impairs the interaction with CUL4 (CUL4A or CUL4B) and formation of DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

Damaged DNA binding protein (DDB) is a heterodimer composed of two subunits, p127 and p48, which are designated DDB1 and DDB2, respectively. The DDB heterodimer is involved in repairing DNA damaged by ultraviolet light. Specifically, DDB, also designated UV-damaged DNA binding protein (UV-DDB), xeroderma pigmentosum group E binding factor (XPE-BF) and hepatitis B virus X-associated protein 1 (XAP-1), binds to damaged cyclobutane pyrimidine dimers (CPDs). Mutations in the DDB2 gene are implicated as causes of xeroderma pigmentosum group E, an autosomal recessive disease in which patients are defective in nucleotide excision DNA repair. XPE is characterized by hypersensitivity of the skin to sunlight with a high frequency of skin cancer as well as neurologic abnormalities. The hepatitis B virus (HBV) X protein interacts with DDB1, which may mediate HBx transactivation.