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PRODUCT CODE: ET7107-26

Recombinant Dcp1a Monoclonal Antibody (ET7107-26)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

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Western blot analysis of Dcp1a on human liver tissue lysates using anti-Dcp1a antibody at 1/500 dilution.
  • Western blot analysis of Dcp1a on human liver tissue lysates using anti-Dcp1a antibody at 1/500 dilution.
  • ICC staining Dcp1a in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Dcp1a in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Dcp1a in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Dcp1a antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Dcp1a antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of LOVO cells with Dcp1a antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Dcp1a on human liver tissue lysates using anti-Dcp1a antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Dcp1a Monoclonal Antibody (ET7107-26)

Immunogen

Recombinant protein within c terminal human dcp1a.

Host

Rabbit

Positive Control

LOVO, SH-SY-5Y, SiHa, human liver tissue, human placenta tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB51-34

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

63 kDa(Predicted band size)

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

Q9NPI6

PROTEIN NAME

Dcp1a

SYNONYMS

DCP1 decapping enzyme homolog A antibody; Dcp1a antibody; DCP1A_HUMAN antibody; Decapping enzyme hDcp1a antibody; Decapping mRNA 1A antibody; HSA275986 antibody; mRNA decapping enzyme 1A antibody; mRNA-decapping enzyme 1A antibody; Nbla00360 antibody; Putative protein product of Nbla00360 antibody; Smad4 interacting transcriptional co activator antibody; Smad4-interacting transcriptional co-activator antibody; Smad4-interacting transcriptional co-activator antibody; SMAD4IP1 antibody SMIF antibody; Transcription factor SMIF antibody

SEQUENCE SIMILARITIES

Belongs to the DCP1 family.

TISSUE SPECIFICITY

Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

Cleavage of the 5'-cap structure is involved in the major 5'-to-3' and nonsense-mediated mRNA decay pathways. The protein complex consisting of Dcp1 and Dcp2 has been identified as the species responsible for the decapping reaction in Saccharomyces cerevisiae. In nonsense-mediated decay, the human decapping complex, made up of S. cerevisiae homologs Dcp1a and hDcp2, may be recruited to mRNAs containing premature termination codons by nonsense-mediated decay factor (Upf) proteins. hDcp2 specifically hydrolyzes methylated capped RNA to release m(7)GDP, thereby aiding in mRNA degradation. Both Dcp1a and hDcp2 colocalize in the cytoplasm. In addition, Dcp1a interacts with Smad4 forming a complex with TGFβ and BMP-4. Dcp1a and Smad4 interact directly through a EVH1/WH1 domain on Dcp1a and a proline-rich activation domain on Smad4. Smad4 is essential to nuclear translocation of Dcp1a as deletion of the Smad4-interacting domain (located in the N-terminal 100 amino acids) of Dcp1a eliminates TGFβ-induced nuclear translocation of Dcp1a.