Applications
-
WB
-
ICC
-
IHC-P
-
FC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant Dcp1a Monoclonal Antibody (ET7107-26)
Immunogen
Recombinant protein within c terminal human dcp1a.
Host
Rabbit
Positive Control
LOVO, SH-SY-5Y, SiHa, human liver tissue, human placenta tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JB51-34
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
63 kDa(Predicted band size)
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:2000
-
ICC
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
Dcp1a
SYNONYMS
DCP1 decapping enzyme homolog A antibody; Dcp1a antibody; DCP1A_HUMAN antibody; Decapping enzyme hDcp1a antibody; Decapping mRNA 1A antibody; HSA275986 antibody; mRNA decapping enzyme 1A antibody; mRNA-decapping enzyme 1A antibody; Nbla00360 antibody; Putative protein product of Nbla00360 antibody; Smad4 interacting transcriptional co activator antibody; Smad4-interacting transcriptional co-activator antibody; Smad4-interacting transcriptional co-activator antibody; SMAD4IP1 antibody SMIF antibody; Transcription factor SMIF antibody
SEQUENCE SIMILARITIES
Belongs to the DCP1 family.
TISSUE SPECIFICITY
Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas.
SUBCELLULAR LOCATION
Cytoplasm. Nucleus.
FUNCTION
Cleavage of the 5'-cap structure is involved in the major 5'-to-3' and nonsense-mediated mRNA decay pathways. The protein complex consisting of Dcp1 and Dcp2 has been identified as the species responsible for the decapping reaction in Saccharomyces cerevisiae. In nonsense-mediated decay, the human decapping complex, made up of S. cerevisiae homologs Dcp1a and hDcp2, may be recruited to mRNAs containing premature termination codons by nonsense-mediated decay factor (Upf) proteins. hDcp2 specifically hydrolyzes methylated capped RNA to release m(7)GDP, thereby aiding in mRNA degradation. Both Dcp1a and hDcp2 colocalize in the cytoplasm. In addition, Dcp1a interacts with Smad4 forming a complex with TGFβ and BMP-4. Dcp1a and Smad4 interact directly through a EVH1/WH1 domain on Dcp1a and a proline-rich activation domain on Smad4. Smad4 is essential to nuclear translocation of Dcp1a as deletion of the Smad4-interacting domain (located in the N-terminal 100 amino acids) of Dcp1a eliminates TGFβ-induced nuclear translocation of Dcp1a.