PRODUCT CODE: ET7107-26

Recombinant Dcp1a Monoclonal Antibody (ET7107-26)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of Dcp1a on human liver tissue lysates using anti-Dcp1a antibody at 1/500 dilution.
  • Western blot analysis of Dcp1a on human liver tissue lysates using anti-Dcp1a antibody at 1/500 dilution.
  • ICC staining Dcp1a in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Dcp1a in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Dcp1a in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Dcp1a antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Dcp1a antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of LOVO cells with Dcp1a antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Dcp1a on human liver tissue lysates using anti-Dcp1a antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Dcp1a Monoclonal Antibody (ET7107-26)

Immunogen

Recombinant protein within c terminal human dcp1a.

Host

Rabbit

Positive Control

LOVO, SH-SY-5Y, SiHa, human liver tissue, human placenta tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB51-34

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

63 kDa(Predicted band size)

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

mRNA-decapping enzyme 1A

GENE NAME

DCP1A

SYNONYMS

DCP1A, SMIF

SEQUENCE SIMILARITIES

Belongs to the DCP1 family.

TISSUE SPECIFICITY

Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas.

SUBCELLULAR LOCATION

Cytoplasm, P-body. Nucleus. Note=Co-localizes with NANOS3 in the processing bodies (By similarity). Predominantly cytoplasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4.

FUNCTION

Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.