PRODUCT CODE: ET1608-23

Recombinant DARPP32 Monoclonal Antibody (ET1608-23)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of DARPP32 on mouse brain lysates using anti-DARPP32 antibody at 1/1,000 dilution.
  • Western blot analysis of DARPP32 on mouse brain lysates using anti-DARPP32 antibody at 1/1,000 dilution.
  • ICC staining DARPP32 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DARPP32 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DARPP32 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-DARPP32 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-DARPP32 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with DARPP32 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of DARPP32 on mouse brain lysates using anti-DARPP32 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant DARPP32 Monoclonal Antibody (ET1608-23)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SH-SY-5Y, MCF-7, mouse brain tissue, rat brain tissue, human gastric carcinoma tissue, human breast carcinoma tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SU0329

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

32 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

DARPP32

SYNONYMS

DARPP 32 antibody; DARPP-32 antibody; Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody; Dopamine and cAMP regulated neuronal phosphoprotein antibody; Dopamine and cAMP regulated phosphoprotein antibody; Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody; Dopamine and cAMP regulated phosphoprotein DARPP32 antibody; Dopamine- and cAMP-regulated neuronal phosphoprotein antibody; FLJ20940 antibody; IPPD antibody; Neuronal phosphoprotein DARPP 32 antibody; PPP1R1B antibody; PPR1B_HUMAN antibody; Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody; Protein phosphatase 1 regulatory inhibitor subunit 1B antibody; Protein phosphatase 1 regulatory subunit 1B antibody

SEQUENCE SIMILARITIES

Belongs to the protein phosphatase inhibitor 1 family.

POST-TRANSLATIONAL MODIFICATION

Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.; Phosphorylation of Thr-34 is required for activity.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Dopaminergic signaling pathways, which are essential for multiple brain functions, are abnormal in several neurological disorders, such as schizophrenia, Parkinson's disease and drug abuse. DARPP-32 (for dopamine and adenosine 3',5'-monophosphate-regulated phosphoprotein) is abundant in neurons that receive dopaminergic input. Activation of PKA and the consequent phosphorylation of DARPP-32 on threonine occurs in response to dopamine acting upon D1-like receptors. Dopamine interaction with D2-like receptors results in the inhibition of PKA activation, the activation of protein phosphatase 2B and the consequent dephosphorylation of DARPP-32. Neurotransmitters other than dopamine may also be able to stimulate the phosphorylation or dephosphorylation of DARPP-32. Phosphorylated DARPP-32 is a potent inhibitor of PP-1.