Lane 1: Hela
Lane 2: MCF-7
Lane 3: A431
Recombinant Rabbit monoclonal primary
Recombinant Cytokeratin 8 Monoclonal Antibody (ET1608-32)
Hela cell lysate, MCF-7 cell lysate, A431 cell lysate, Hela, MCF-7, A431, human liver tissue, human breast carcinoma tissue.
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A affinity purified.
CARD2 antibody; Cell proliferation inducing gene 46 protein antibody; Cell proliferation inducing protein 46 antibody; CK 8 antibody; CK-8 antibody; CK8 antibody; CYK8 antibody; Cytokeratin 8 antibody; Cytokeratin-8 antibody; K2C8 antibody; K2C8_HUMAN antibody; K8 antibody; Keratin 8 antibody; Keratin antibody; keratin type II cytoskeletal 8 antibody; Keratin-8 antibody; KRT8 antibody; type II cytoskeletal 8 antibody; Type-II keratin Kb8 antibody
Belongs to the intermediate filament family.
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.; Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.; O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.
Nucleoplasm, Nucleus matrix, Cytoplasm.
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. They have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratin 8 expression is seen in epithelium and epithelium-derived tumors. The Cytokeratin 8 and 18 pair are normally expressed in simple epithelia, but not in stratified epithelial cells. Research indicates that squamous cell carcinomas derived from stratified epithelia show abnormal expression of Cytokeratin 8 and 18, although it is not known whether these proteins contribute to the malignant phenotype of the cells. Expression of Cytokeratin 8 and 18 in oral squamous cell carcinomas is an independent prognostic marker that indicates a poor prognosis. Cytokeratin 8 expression correlates with malignancy in leukoplakia and carcinomas of the head and neck; it is expressed in all non-small-cell lung cancers. Cytokeratin 8 has been shown to possess extracellular epitopes on tumor cells, which may represent valuable targets for therapy.