PRODUCT CODE: ET1608-32

Recombinant Cytokeratin 8 Monoclonal Antibody (ET1608-32)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Cytokeratin 8 on different lysates using anti-Cytokeratin 8 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
  Lane 1: Hela <br />
  Lane 2: MCF-7 <br />
  Lane 3: A431
  • Western blot analysis of Cytokeratin 8 on different lysates using anti-Cytokeratin 8 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
  Lane 1: Hela <br />
  Lane 2: MCF-7 <br />
  Lane 3: A431
  • ICC staining Cytokeratin 8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Cytokeratin 8 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Cytokeratin 8 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with Cytokeratin 8 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Cytokeratin 8 on different lysates using anti-Cytokeratin 8 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: MCF-7
Lane 3: A431

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Cytokeratin 8 Monoclonal Antibody (ET1608-32)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela cell lysate, MCF-7 cell lysate, A431 cell lysate, Hela, MCF-7, A431, human liver tissue, human breast carcinoma tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SU0338

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

53 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Cytokeratin 8

SYNONYMS

CARD2 antibody; Cell proliferation inducing gene 46 protein antibody; Cell proliferation inducing protein 46 antibody; CK 8 antibody; CK-8 antibody; CK8 antibody; CYK8 antibody; Cytokeratin 8 antibody; Cytokeratin-8 antibody; K2C8 antibody; K2C8_HUMAN antibody; K8 antibody; Keratin 8 antibody; Keratin antibody; keratin type II cytoskeletal 8 antibody; Keratin-8 antibody; KRT8 antibody; type II cytoskeletal 8 antibody; Type-II keratin Kb8 antibody

SEQUENCE SIMILARITIES

Belongs to the intermediate filament family.

TISSUE SPECIFICITY

Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.; Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.; O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.

SUBCELLULAR LOCATION

Nucleoplasm, Nucleus matrix, Cytoplasm.

FUNCTION

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. They have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratin 8 expression is seen in epithelium and epithelium-derived tumors. The Cytokeratin 8 and 18 pair are normally expressed in simple epithelia, but not in stratified epithelial cells. Research indicates that squamous cell carcinomas derived from stratified epithelia show abnormal expression of Cytokeratin 8 and 18, although it is not known whether these proteins contribute to the malignant phenotype of the cells. Expression of Cytokeratin 8 and 18 in oral squamous cell carcinomas is an independent prognostic marker that indicates a poor prognosis. Cytokeratin 8 expression correlates with malignancy in leukoplakia and carcinomas of the head and neck; it is expressed in all non-small-cell lung cancers. Cytokeratin 8 has been shown to possess extracellular epitopes on tumor cells, which may represent valuable targets for therapy.