PRODUCT CODE: ET1611-70

Recombinant Cytokeratin 6 Monoclonal Antibody (ET1611-70)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of Cytokeratin 6 on human skin lysates using anti-Cytokeratin 6 antibody at 1/1,000 dilution.
  • Western blot analysis of Cytokeratin 6 on human skin lysates using anti-Cytokeratin 6 antibody at 1/1,000 dilution.
  • ICC staining Cytokeratin 6 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 6 antibody. Counter stained with hematoxylin.
Western blot analysis of Cytokeratin 6 on human skin lysates using anti-Cytokeratin 6 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Cytokeratin 6 Monoclonal Antibody (ET1611-70)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, human tonsil tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN71-07

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

60 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Cytokeratin 6

SYNONYMS

CK 6A antibody; CK 6B antibody; CK 6C antibody; CK 6D antibody; CK 6E antibody; CK-6C antibody; CK-6E antibody; Cytokeratin 6a antibody; Cytokeratin 6B antibody; Cytokeratin 6C antibody; Cytokeratin 6D antibody; Cytokeratin 6E antibody; Cytokeratin-6C antibody; Cytokeratin-6E antibody; K2C6C_HUMAN antibody; K6a keratin antibody; K6b keratin antibody; K6C antibody; K6c keratin antibody; K6d keratin antibody; K6e keratin antibody; Keratin antibody; Keratin K6h antibody; Keratin type II cytoskeletal 6A antibody; Keratin type II cytoskeletal 6B antibody; Keratin type II cytoskeletal 6C antibody; Keratin type II cytoskeletal 6D antibody; Keratin type II cytoskeletal 6E antibody; Keratin-6C antibody; KRT6A antibody; KRT6B antibody; KRT6C antibody; KRT6D antibody; KRT6E antibody; type II cytoskeletal 6C antibody; Type-II keratin Kb12 antibody

SEQUENCE SIMILARITIES

Belongs to the intermediate filament family.

TISSUE SPECIFICITY

Expressed in the corneal epithelium (at protein level).

FUNCTION

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The alpha-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins, which are useful markers of tissue differentiation, also aid in the characterization of malignant tumors. IL-1 and TNFα induce transcription of cytokeratin 6 in epidermal keratinocytes via the C/EBP β transcription factor. In humans, multiple isoforms of cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies.