PRODUCT CODE: ET1605-17

Recombinant Cytokeratin 5 Monoclonal Antibody (ET1605-17)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Cytokeratin 5 on A431 cell lysates using anti-Cytokeratin 5 at 1/500 dilution.
  • Western blot analysis of Cytokeratin 5 on A431 cell lysates using anti-Cytokeratin 5 at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Cytokeratin 5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human brest cancer tissue using anti-Cytokeratin 5 antibody. Counter stained with hematoxylin.
Western blot analysis of Cytokeratin 5 on A431 cell lysates using anti-Cytokeratin 5 at 1/500 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Cytokeratin 5 Monoclonal Antibody (ET1605-17)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, human tonsil tissue, human lung cancer tissue, human breast cancer tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SY88-19

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

62 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Cytokeratin 5

SYNONYMS

58 kDa cytokeratin antibody; CK-5 antibody; CK5 antibody; Cytokeratin-5 antibody; Cytokeratin5 antibody; DDD antibody; DDD1 antibody; EBS2 antibody; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types antibody; K2C5_HUMAN antibody; K5 antibody; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types) antibody; Keratin 5 antibody; Keratin antibody; keratin complex 2, basic, gene 5 antibody; keratin, type II cytoskeletal 5 antibody; Keratin-5 antibody; Keratin5 antibody; KRT 5 antibody; Krt5 antibody; KRT5A antibody; type II cytoskeletal 5 antibody; Type-II keratin Kb5 antibody

SEQUENCE SIMILARITIES

Belongs to the intermediate filament family.

TISSUE SPECIFICITY

Expressed in corneal epithelium (at protein level).

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. Cytokeratin 5 is expressed in normal basal cells. Mutations of the cytokeratin 5 gene (KRT5) have been shown to result in the autosomal dominant disorder epidermolysis bullosa (EB).