PRODUCT CODE: ET1611-55

Recombinant Cytokeratin 13 Monoclonal Antibody (ET1611-55)

  • Zebrafish
  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Zebrafish

Western blot analysis of Cytokeratin 13 on human lung lysates using anti-Cytokeratin 13 antibody at 1/1,000 dilution.
  • Western blot analysis of Cytokeratin 13 on human lung lysates using anti-Cytokeratin 13 antibody at 1/1,000 dilution.
  • ICC staining Cytokeratin 13 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Cytokeratin 13 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Cytokeratin 13 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 13 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 13 antibody. Counter stained with hematoxylin.
  • Western blot analysis of Cytokeratin 13 hybrid fish (crucian-carp) brain tissue lysate using anti-Cytokeratin 13 antibody at 1/500 dilution.
Western blot analysis of Cytokeratin 13 on human lung lysates using anti-Cytokeratin 13 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Zebrafish

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Cytokeratin 13 Monoclonal Antibody (ET1611-55)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human lung tissue lysates, hybrid fish (crucian-carp) brain tissue lysates, Hela, MCF-7, A549, human tonsil tissue, human breast carcinoma tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN71-09

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Cytokeratin 13

SYNONYMS

47 kDa cytokeratin antibody; CK-13 antibody; CK13 antibody; Cytokeratin 13 antibody; Cytokeratin-13 antibody; K13 antibody; K1C13_HUMAN antibody; Ka13 antibody; Keratin 13 antibody; Keratin antibody; keratin type I cytoskeletal 13 antibody; Keratin-13 antibody; Krt-1.13 antibody; Krt1-13 antibody; KRT13 antibody; MGC161462 antibody; MGC3781 antibody; type I cytoskeletal 13 antibody; Type I keratin Ka13 antibody; WSN2 antibody

SEQUENCE SIMILARITIES

Belongs to the intermediate filament family.

TISSUE SPECIFICITY

Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta.

POST-TRANSLATIONAL MODIFICATION

O-glycosylated; glycans consist of single N-acetylglucosamine residues.

SUBCELLULAR LOCATION

Extracellular exosome, intermediate filament cytoskeleton, keratin filament, nucleus.

FUNCTION

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratins 10 and 13 are present in the cytoskeletal region of a subset of squamous cell carcinomas. Cytokeratin 13 belongs to the intermediate filament family and is a heterotetramer of two type I acidic and two type II basic keratins. It is generally associated with cytokeratin 4. Defects in the KRT13 gene are a cause of white sponge nevus of cannon (WSN), a rare autosomal dominant disorder which predominantly affects noncornified stratified squamous epithelia and is characterized by the presence of soft, white and spongy plaques in the oral mucosa.