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PRODUCT CODE: ET1611-46

Recombinant Cytokeratin 1 Monoclonal Antibody (ET1611-46)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Cytokeratin 1 on different lysates using anti-Cytokeratin 1 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: 293T <br /> Lane 2: Hela
  • Western blot analysis of Cytokeratin 1 on different lysates using anti-Cytokeratin 1 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: 293T <br /> Lane 2: Hela
  • Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 1 antibody. Counter stained with hematoxylin.
Western blot analysis of Cytokeratin 1 on different lysates using anti-Cytokeratin 1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293T
Lane 2: Hela

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Cytokeratin 1 Monoclonal Antibody (ET1611-46)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse skin tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN72-08

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

72 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • IHC-P

  • 1:100-1:500

TARGET

UNIPROT #

P04264

PROTEIN NAME

Keratin, type II cytoskeletal 1

GENE NAME

KRT1

SYNONYMS

CK-1, K1, KRT1

SEQUENCE SIMILARITIES

Belongs to the intermediate filament family.

TISSUE SPECIFICITY

The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.

POST-TRANSLATIONAL MODIFICATION

Undergoes deimination of some arginine residues (citrullination).

SUBCELLULAR LOCATION

Cell membrane. Note=Located on plasma membrane of neuroblastoma NMB7 cells.

FUNCTION

May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.