Positive control:
Lane 1: 293T
Lane 2: Hela
Applications
-
WB
-
IHC-P
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant Cytokeratin 1 Monoclonal Antibody (ET1611-46)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Hela cell lysate, 293T cell lysate, mouse skin tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN72-08
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
72 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000
-
IHC-P
-
1:100-1:500
TARGET
UNIPROT #
PROTEIN NAME
Cytokeratin 1
SYNONYMS
67 kDa cytokeratin antibody; CK-1 antibody; CK1 antibody; Cytokeratin-1 antibody; Cytokeratin1 antibody; EHK antibody; EHK1 antibody; Epidermolytic hyperkeratosis 1 antibody; EPPK antibody; Hair alpha protein antibody; K1 antibody; K2C1_HUMAN antibody; Keratin antibody; Keratin type II cytoskeletal 1 antibody; Keratin-1 antibody; Keratin1 antibody; KRT 1 antibody; Krt1 antibody; KRT1A antibody; NEPPK antibody; type II cytoskeletal 1 antibody; Type II keratin Kb1 antibody; Type-II keratin Kb1 antibody
SEQUENCE SIMILARITIES
Belongs to the intermediate filament family.
TISSUE SPECIFICITY
The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.
POST-TRANSLATIONAL MODIFICATION
Undergoes deimination of some arginine residues (citrullination).
SUBCELLULAR LOCATION
Cell membrane.
FUNCTION
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins constitute up to 85% of a mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization, and they function to maintain the overall structural integrity of epithelial cells. The alpha-helical coiled-coil dimers associate laterally end-to-end to form 10-nm diameter filaments. Cytokeratins are useful markers of tissue differentiation, and they aid in the characterization of malignant tumors. Cytokeratin 1 is highly expressed in several malignancies including epithelioid hemangioendohtheliomas, angiosarcomas, schwannomas, epithelioid sarcomas and synodal sarcomas. The gene encoding human Cytokeratin 1 maps to chromosome 12q13.13. Mutations in the gene encoding human Cytokeratin 1 lead to abnormal filament associations and epidermolytic hyperkeratosis.