PRODUCT CODE: ET1611-46

Recombinant Cytokeratin 1 Monoclonal Antibody (ET1611-46)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Cytokeratin 1 on different lysates using anti-Cytokeratin 1 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: 293T <br />
 Lane 2: Hela
  • Western blot analysis of Cytokeratin 1 on different lysates using anti-Cytokeratin 1 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: 293T <br />
 Lane 2: Hela
  • Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 1 antibody. Counter stained with hematoxylin.
Western blot analysis of Cytokeratin 1 on different lysates using anti-Cytokeratin 1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293T
Lane 2: Hela

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Cytokeratin 1 Monoclonal Antibody (ET1611-46)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela cell lysate, 293T cell lysate, mouse skin tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN72-08

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

72 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • IHC-P

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

Cytokeratin 1

SYNONYMS

67 kDa cytokeratin antibody; CK-1 antibody; CK1 antibody; Cytokeratin-1 antibody; Cytokeratin1 antibody; EHK antibody; EHK1 antibody; Epidermolytic hyperkeratosis 1 antibody; EPPK antibody; Hair alpha protein antibody; K1 antibody; K2C1_HUMAN antibody; Keratin antibody; Keratin type II cytoskeletal 1 antibody; Keratin-1 antibody; Keratin1 antibody; KRT 1 antibody; Krt1 antibody; KRT1A antibody; NEPPK antibody; type II cytoskeletal 1 antibody; Type II keratin Kb1 antibody; Type-II keratin Kb1 antibody

SEQUENCE SIMILARITIES

Belongs to the intermediate filament family.

TISSUE SPECIFICITY

The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.

POST-TRANSLATIONAL MODIFICATION

Undergoes deimination of some arginine residues (citrullination).

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins constitute up to 85% of a mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization, and they function to maintain the overall structural integrity of epithelial cells. The alpha-helical coiled-coil dimers associate laterally end-to-end to form 10-nm diameter filaments. Cytokeratins are useful markers of tissue differentiation, and they aid in the characterization of malignant tumors. Cytokeratin 1 is highly expressed in several malignancies including epithelioid hemangioendohtheliomas, angiosarcomas, schwannomas, epithelioid sarcomas and synodal sarcomas. The gene encoding human Cytokeratin 1 maps to chromosome 12q13.13. Mutations in the gene encoding human Cytokeratin 1 lead to abnormal filament associations and epidermolytic hyperkeratosis.