PRODUCT CODE: ET1705-28

Recombinant Cytochrome P450 2D6 Monoclonal Antibody (ET1705-28)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of Cytochrome P450 2D6 on HepG2 cell using anti-Cytochrome P450 2D6 antibody at 1/1,000 dilution.
  • Western blot analysis of Cytochrome P450 2D6 on HepG2 cell using anti-Cytochrome P450 2D6 antibody at 1/1,000 dilution.
  • ICC staining Cytochrome P450 2D6 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Cytochrome P450 2D6 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytochrome P450 2D6 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cytochrome P450 2D6 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SW480 cells with Cytochrome P450 2D6 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of Cytochrome P450 2D6 on HepG2 cell using anti-Cytochrome P450 2D6 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Cytochrome P450 2D6 Monoclonal Antibody (ET1705-28)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HepG2, SW480, human liver tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM44-23

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

56 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Cytochrome P450 2D6

SYNONYMS

CPD6 antibody; CYP2D antibody; CYP2D6 antibody; CYP2D7AP antibody; CYP2D7BP antibody; CYP2D7P2 antibody; CYP2D8P2 antibody; CYP2DL1 antibody; CYPIID6 antibody; Cytochrome P450 DB1 antibody; Cytochrome P450 family 2 subfamily D member 6 antibody; Cytochrome P450 family 2 subfamily D polypeptide 6 antibody; Debrisoquine 4 hydroxylase antibody; Flavoprotein linked monooxygenase antibody; Microsomal monooxygenase antibody; P450 DB1 antibody; P450C2D antibody; P450DB1 antibody; Xenobiotic monooxygenase antibody

SEQUENCE SIMILARITIES

Belongs to the cytochrome P450 family.

SUBCELLULAR LOCATION

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

FUNCTION

The P450II family comprises at least five subfamilies, designated A through E by the system of nomenclature recommended by an international committee. The P450IID subfamily comprises at least two genes in the rat, one of which is highly specific for debrisoquine 4-hydroxylase activity. An association of this gene with lung cancer has been found. Enhanced CYP2D6 activity has been related to malignancies of the bladder, liver, pharynx and stomach, and especially to cigarette-smoking-induced lung cancer. The data suggests that enhanced CYP2D6-mediated metabolism of one or more dietary and other environmental agents, to form a reactive intermediate, plays a role in cancer initiation and/or promotion in various tissues. CYP2D6 polymorphism, which is responsible for the variation in metabolism of debrisoquine 4-hydroxylase, is important in the metabolism of more than 30 drugs and environmental chemicals, including as much as 20% of all commonly prescribed drugs. The gene which encodes CYP2D6 maps to human chromosome 22q13.1.