PRODUCT CODE: ET1701-72

Recombinant Cystatin C Monoclonal Antibody (ET1701-72)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Cystatin C on different lysates using anti-Cystatin C antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: Mouse spleen
  • Western blot analysis of Cystatin C on different lysates using anti-Cystatin C antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: Mouse spleen
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Cystatin C antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cystatin C antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Cystatin C antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Cystatin C antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Cystatin C antibody. Counter stained with hematoxylin.
Western blot analysis of Cystatin C on different lysates using anti-Cystatin C antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Mouse spleen

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Cystatin C Monoclonal Antibody (ET1701-72)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, human liver cancer tissue, human kidney tissue, mouse brain tissue, mouse spleen tissue, mouse placenta tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ09-16

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

16 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Cystatin-C

GENE NAME

CST3

SYNONYMS

CST3

SEQUENCE SIMILARITIES

Belongs to the cystatin family.

TISSUE SPECIFICITY

Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.

POST-TRANSLATIONAL MODIFICATION

The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.