PRODUCT CODE: ET1705-7

Recombinant Cyclophilin F Monoclonal Antibody (ET1705-7)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Cyclophilin F on human heart (1) and human brain (2) tissue lysate using anti-Cyclophilin F antibody at 1/1,000 dilution.
  • Western blot analysis of Cyclophilin F on human heart (1) and human brain (2) tissue lysate using anti-Cyclophilin F antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cyclophilin F antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Cyclophilin F antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Cyclophilin F antibody. Counter stained with hematoxylin.
Western blot analysis of Cyclophilin F on human heart (1) and human brain (2) tissue lysate using anti-Cyclophilin F antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Cyclophilin F Monoclonal Antibody (ET1705-7)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human heart tissue lysate, human brain tissue lysate, human colon cancer tissue, human breast cancer tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM71-39

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

20 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Peptidyl-prolyl cis-trans isomerase F, mitochondrial

GENE NAME

PPIF

SYNONYMS

PPIase F, CyP-D, CypD, CyP-M, PPIF, CYP3

SEQUENCE SIMILARITIES

Belongs to the cyclophilin-type PPIase family.

POST-TRANSLATIONAL MODIFICATION

Deacetylated at Lys-167 by SIRT3.

SUBCELLULAR LOCATION

Mitochondrion matrix.

FUNCTION

PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. Involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated. In cooperation with mitochondrial TP53 is involved in activating oxidative stress-induced necrosis. Involved in modulation of mitochondrial membrane F(1)F(0) ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels. Has anti-apoptotic activity independently of mPTP and in cooperation with BCL2 inhibits cytochrome c-dependent apoptosis.