PRODUCT CODE: ET1705-7

Recombinant Cyclophilin F Monoclonal Antibody (ET1705-7)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Cyclophilin F on human heart (1) and human brain (2) tissue lysate using anti-Cyclophilin F antibody at 1/1,000 dilution.
  • Western blot analysis of Cyclophilin F on human heart (1) and human brain (2) tissue lysate using anti-Cyclophilin F antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cyclophilin F antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Cyclophilin F antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Cyclophilin F antibody. Counter stained with hematoxylin.
Western blot analysis of Cyclophilin F on human heart (1) and human brain (2) tissue lysate using anti-Cyclophilin F antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Cyclophilin F Monoclonal Antibody (ET1705-7)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human heart tissue lysate, human brain tissue lysate, human colon cancer tissue, human breast cancer tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM71-39

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

20 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Cyclophilin F

SEQUENCE SIMILARITIES

Belongs to the cyclophilin-type PPIase family.

POST-TRANSLATIONAL MODIFICATION

Deacetylated at Lys-167 by SIRT3.

SUBCELLULAR LOCATION

Mitochondrion matrix.

FUNCTION

Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins. Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of cyclosporin A (CsA) and is encoded by a single unique gene conserved between yeast to humans. Cyclophilin B (CyPB) is secreted in biological fluids such as blood or milk, and binds to a specific receptor present on human peripheral blood lymphocytes and expressed in Jurkat cells, a line of human lymphoblasts. Cyclophilin D (CyP40) is a widely expressed cytoplasmic protein that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Cyclophilin F (CyP3), is also known as PPIF, Cyp-D or Rotamase F, is a 207 amino acid mitochondrial matrix protein involved in the induction of necrotic and apoptotic cell death through the activation of the mitochondrial permeability transition (mPT) pore.