Positive control:
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: NIH/3T3 cell lysate
Applications
-
WB
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant Cyclophilin B Monoclonal Antibody (ET1609-58)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Hela cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, rat epididymis tissue, human thyroid tissue, human placenta tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
ST05-22
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
18 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:5,000
TARGET
UNIPROT #
PROTEIN NAME
Cyclophilin B
SYNONYMS
AA408962 antibody; AA553318 antibody; AI844835 antibody; Cphn 2 antibody; Cphn2 antibody; Cyclophilin B antibody; Cyclophilin like protein antibody; CyP 20b antibody; CYP S1 antibody; CYP-S1 antibody; CYPB antibody; EC 5.2.1.8 antibody; MGC14109 antibody; MGC2224 antibody; OI9 antibody; peptidyl prolyl cis trans isomerase B antibody; Peptidyl prolyl cis trans isomerase B precursor antibody; Peptidyl-prolyl cis-trans isomerase B antibody; peptidylprolyl isomerase B (cyclophilin B) antibody; Peptidylprolyl isomerase B antibody; PPIase antibody; PPIase B antibody; Ppib antibody; PPIB_HUMAN antibody; Rotamase antibody; Rotamase B antibody; S cyclophilin antibody; S-cyclophilin antibody; SCYLP antibody
SEQUENCE SIMILARITIES
Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.
SUBCELLULAR LOCATION
Endoplasmic reticulum lumen, Melanosome.
FUNCTION
The immunosuppressant cyclosporin A (CsA) forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins. Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of the Cyclosporin A and is encoded by a single unique gene conserved from yeast to humans. CyPA is known for its involvement in T cell differentiation and proliferation and is highly expressed in brain. CyPA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication. Cyclophilin B (CyPB) is a member of the cyclophilin family with specific N- and C-terminal extensions. Unlike CyPA, CyPB has a signal sequence leading to its translocation in the endoplasmic reticulum. CyPB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes.