Lane 1: Hela
Lane 2: K562
Recombinant Rabbit monoclonal primary
Recombinant Cyclin B2 Monoclonal Antibody (ET1612-21)
K562 cell lysate, Hela cell lysate, PC-12, mouse testis tissue, mouse colon tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A affinity purified.
ccnb2 antibody; CCNB2_HUMAN antibody; CycB2 antibody; Cyclin B2 antibody; G2 mitotic specific cyclin B2 antibody; G2/mitotic specific cyclin B2 antibody; G2/mitotic-specific cyclin-B2 antibody; HsT17299 antibody; MGC108931 antibody; MGC140694 antibody
Belongs to the cyclin family. Cyclin AB subfamily.
Accumulates steadily during G2 and is abruptly destroyed at mitosis.
Centrosome, microtubule cytoskeleton, cytosol, nucleus, cyclin-dependent protein kinase holoenzyme complex, cytoplasm, host cell nucleus, membrane.
In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M-phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc 1). The Cdc/cyclin enzyme is subject to multiple levels of control of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B enzyme and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex . Evidence has established that B-type cyclins not only act on M-phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B-type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions.