PRODUCT CODE: ET1612-21

Recombinant Cyclin B2 Monoclonal Antibody (ET1612-21)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Cyclin B2 on different lysates using anti-Cyclin B2 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: K562
  • Western blot analysis of Cyclin B2 on different lysates using anti-Cyclin B2 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: K562
  • ICC staining Cyclin B2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Cyclin B2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cyclin B2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Cyclin B2 antibody. Counter stained with hematoxylin.
Western blot analysis of Cyclin B2 on different lysates using anti-Cyclin B2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: K562

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Cyclin B2 Monoclonal Antibody (ET1612-21)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

K562, PC-12, Hela, mouse testis tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD2045

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

45 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

G2/mitotic-specific cyclin-B2

GENE NAME

CCNB2

SYNONYMS

CCNB2

SEQUENCE SIMILARITIES

Belongs to the cyclin family. Cyclin AB subfamily.

DEVELOPMENTAL STAGE

Accumulates steadily during G2 and is abruptly destroyed at mitosis.

FUNCTION

Essential for the control of the cell cycle at the G2/M (mitosis) transition.