Lane 1: Hela cell lysate
Lane 2: Daudi cell lysate
Lane 3: K562 cell lysate
Recombinant Rabbit monoclonal primary
Recombinant Cyclin B1 Monoclonal Antibody (ET1608-27)
HUVEC, MCF-7, Hela, HepG2, human liver tissue, mouse liver tissue, mouse brain tissue, human lung tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A purified.
Belongs to the cyclin family. Cyclin AB subfamily.
Accumulates steadily during G2 and is abruptly destroyed at mitosis.
Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.; Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Essential for the control of the cell cycle at the G2/M (mitosis) transition.