PRODUCT CODE: ET1612-8

Recombinant Cyclin A1+ Cyclin A2 Monoclonal Antibody (ET1612-8)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

Western blot analysis of Cyclin A1/A2 on different lysates using anti-Cyclin A1/A2 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: HepG2
  • Western blot analysis of Cyclin A1/A2 on different lysates using anti-Cyclin A1/A2 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: HepG2
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cyclin A1/A2 antibody. Counter stained with hematoxylin.
Western blot analysis of Cyclin A1/A2 on different lysates using anti-Cyclin A1/A2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Cyclin A1+ Cyclin A2 Monoclonal Antibody (ET1612-8)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, HepG2, human colon cancer tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD2053

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

52/49 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Cyclin A1+ Cyclin A2

SYNONYMS

CCN1 antibody; CCNA antibody; CCNA1 antibody; CCNA2 antibody; CT146 antibody; Cyclin-A antibody; Cyclin-A1 antibody; Cyclin-A2 antibody

SEQUENCE SIMILARITIES

Belongs to the cyclin family. Cyclin AB subfamily.

DEVELOPMENTAL STAGE

Accumulates steadily during G2 and is abruptly destroyed at mitosis. Not detected during the G1 phase of the cell cycle. It accumulates during the DNA synthesis/S phase and disappears as cells progress into mitosis, between prophase and metaphase (at protein level).

POST-TRANSLATIONAL MODIFICATION

Polyubiquitinated via 'Lys-11'-linked ubiquitin by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome. Deubiquitinated and stabilized by USP37 enables entry into S phase.

SUBCELLULAR LOCATION

Nucleus, Cytoplasm.

FUNCTION

The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin-dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F, and retinoblastoma protein p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34. Cyclin A2 is a member of the highly conserved cyclin family. Cyclins regulate CDK kinases and different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin A2 is expressed in all tissues tested, in contrast to cyclin A1, which is present only in germ cells. The Cyclin A2 protein binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. Cyclin A2 also functions in the transition to DNA replication and synthesis phases of the cell cycle and is quickly destroyed as the cell moves into mitosis.