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PRODUCT CODE: ET7106-56

Recombinant Cullin 4a Monoclonal Antibody (ET7106-56)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

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Western blot analysis of Cullin 4a on Hela cell lysates using anti-Cullin 4a antibody at 1/500 dilution.
  • Western blot analysis of Cullin 4a on Hela cell lysates using anti-Cullin 4a antibody at 1/500 dilution.
  • ICC staining Cullin 4a in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Cullin 4a in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human cervix tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with Cullin 4a antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Cullin 4a on Hela cell lysates using anti-Cullin 4a antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Cullin 4a Monoclonal Antibody (ET7106-56)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, A431, HUVEC, human liver cance tissue, human breast cancer tissue, human colon cancer tissue, human kidney tissue, human cervix tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU07-33

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

88 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

Q13619

PROTEIN NAME

Cullin 4a

SYNONYMS

2810470J21Rik antibody; AW495282 antibody; CUL 4A antibody; CUL-4A antibody; Cul4a antibody; Cul4a protein antibody; CUL4A_HUMAN antibody; Cullin-4A antibody; MGC36573 antibody; MGC64071 antibody

SEQUENCE SIMILARITIES

Belongs to the cullin family.

POST-TRANSLATIONAL MODIFICATION

Neddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.; (Microbial infection) Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled (H3.1) and replication-independent (H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition.