PRODUCT CODE: ET1705-82

Recombinant Cullin 1 Monoclonal Antibody (ET1705-82)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

  • FC

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Cullin 1 on MCF-7 cell using anti-Cullin 1 antibody at 1/1,000 dilution.
  • Western blot analysis of Cullin 1 on MCF-7 cell using anti-Cullin 1 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Cullin 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Cullin 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse oviduct tissue using anti-Cullin 1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with Cullin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of Cullin 1 on MCF-7 cell using anti-Cullin 1 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • IP

  • FC

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Cullin 1 Monoclonal Antibody (ET1705-82)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

MCF-7, Hela, rat brain tissue, human stomach cancer tissue, mouse oviduct tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM72-30

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50 ICC/IF

TARGET

UNIPROT #

PROTEIN NAME

Cullin 1

SYNONYMS

CUL 1 antibody; CUL-1 antibody; CUL1 antibody; CUL1_HUMAN antibody; Cullin-1 antibody; Cullin1 antibody; MGC149834 antibody; MGC149835 antibody

SEQUENCE SIMILARITIES

Belongs to the cullin family.

TISSUE SPECIFICITY

Expressed in lung fibroblasts.

POST-TRANSLATIONAL MODIFICATION

Neddylated; which enhances the ubiquitination activity of SCF and prevents binding of the inhibitor CAND1. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.; (Microbial infection) Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome.

SUBCELLULAR LOCATION

Cytoplasm, Nucleoplasm.

FUNCTION

Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, also designated vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.