PRODUCT CODE: ET1703-90

Recombinant CTCF Monoclonal Antibody (ET1703-90)

  • Zebrafish
  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

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Western blot analysis of CTCF on different cell lysates using anti-CTCF antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: 293T<br />
 Lane 2: MCF-7
  • Western blot analysis of CTCF on different cell lysates using anti-CTCF antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: 293T<br />
 Lane 2: MCF-7
  • ICC staining CTCF in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CTCF in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CTCF in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CTCF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human endometrium tissue using anti-CTCF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-CTCF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CTCF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CTCF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CTCF antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of 293T cells with CTCF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
  • Western blot analysis of CTCF on Zebrafish tissue lysates using anti-CTCF antibody at 1/200 dilution.
  • Western blot analysis of CTCF on hybrid fish (crucian-carp) brain tissue lysate using anti-CTCF antibody at 1/500 dilution.
Western blot analysis of CTCF on different cell lysates using anti-CTCF antibody at 1/500 dilution.
Positive control:
Lane 1: 293T
Lane 2: MCF-7

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant CTCF Monoclonal Antibody (ET1703-90)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

MCF-7 cell lysates, zebrafish tissue lysates, hybrid fish (crucian-carp) brain tissue lysates, Hela, MCF-7, NIH/3T3, human breast carcinoma tissue, human endometrium tissue, mouse stomach tissue, human liver tissue, human kidney tissue, mouse colon tissue, 293T.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-61

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

140/83 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

CTCF

SYNONYMS

11 zinc finger protein antibody; 11 zinc finger transcriptional repressor antibody; 11-zinc finger protein antibody; CCCTC binding factor (zinc finger protein) antibody; CCCTC binding factor antibody; CCCTC-binding factor antibody; Ctcf antibody; CTCF_HUMAN antibody; CTCFL paralog antibody; MRD21 antibody; Transcriptional repressor CTCF antibody

SEQUENCE SIMILARITIES

Belongs to the CTCF zinc-finger protein family.

TISSUE SPECIFICITY

Ubiquitous. Absent in primary spermatocytes.

POST-TRANSLATIONAL MODIFICATION

Sumoylated on Lys-74 and Lys-689; sumoylation of CTCF contributes to the repressive function of CTCF on the MYC P2 promoter.

SUBCELLULAR LOCATION

Nucleoplasm, Chromosome, centromere.

FUNCTION

CTCF belongs to the zinc finger transcription factor family, and it recognizes unusually long and remarkably divergent DNA target sequences to influence expression of many various genes. The DNA-binding domain of CTCF is composed of 11 Zn fingers including 10 that are of C2H2 class, and 1 that is of C2HC class, and they are highly conserved between vertebrate species. CTCF functions as a repressor of the c-myc gene and as a regulator of lysozyme gene expression. In addition, CTCF associates with the essential activator domain in the promotor region of the amyloid beta-protein precursor (APP) gene to activate transcription of APP. Expression of CTCF up-regulates APP expression and thereby, enhances synapse formations between primary neurons during development. CTCF is ubiquitously expressed and localized to the nucleus. During terminal differentiation, CTCF is negatively regulated by differential phosphorylation and also by decreases in CTCF mRNA and protein expression.