PRODUCT CODE: ET1703-90

Recombinant CTCF Monoclonal Antibody (ET1703-90)

  • Zebrafish
  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

Western blot analysis of CTCF on different cell lysates using anti-CTCF antibody at 1/500 dilution.<br />
Positive control:<br />
Lane 1: 293T<br />
Lane 2: MCF-7
  • Western blot analysis of CTCF on different cell lysates using anti-CTCF antibody at 1/500 dilution.<br />
Positive control:<br />
Lane 1: 293T<br />
Lane 2: MCF-7
  • ICC staining CTCF in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CTCF in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CTCF in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CTCF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human endometrium tissue using anti-CTCF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-CTCF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CTCF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CTCF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CTCF antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of 293T cells with CTCF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. <br />
  • Western blot analysis of CTCF on Zebrafish tissue lysates using anti-CTCF antibody at 1/200 dilution.
  • Western blot analysis of CTCF on hybrid fish (crucian-carp) brain tissue lysate using anti-CTCF antibody at 1/500 dilution.
Western blot analysis of CTCF on different cell lysates using anti-CTCF antibody at 1/500 dilution.
Positive control:
Lane 1: 293T
Lane 2: MCF-7

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant CTCF Monoclonal Antibody (ET1703-90)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, 293T, MCF-7, NIH-3T3, human liver tissue, human breast carcinoma tissue, human kidney tissue, human endometrium tissue, mouse colon tissue, mouse stomach tissue, Zebrafish tissue lysates.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-61

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

140/83 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Transcriptional repressor CTCF

GENE NAME

CTCF

SYNONYMS

CTCF

SEQUENCE SIMILARITIES

Belongs to the CTCF zinc-finger protein family.

TISSUE SPECIFICITY

Ubiquitous. Absent in primary spermatocytes.

POST-TRANSLATIONAL MODIFICATION

Sumoylated on Lys-74 and Lys-689; sumoylation of CTCF contributes to the repressive function of CTCF on the MYC P2 promoter.

SUBCELLULAR LOCATION

Nucleus, nucleoplasm. Chromosome. Chromosome, centromere. Note=May translocate to the nucleolus upon cell differentiation. Associates with both centromeres and chromosomal arms during metaphase. Associates with the H19 ICR in mitotic chromosomes. May be preferentially excluded from heterochromatin during interphase.

FUNCTION

Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays an important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19. Plays a role in the recruitment of CENPE to the pericentromeric/centromeric regions of the chromosome during mitosis.