PRODUCT CODE: ET7107-52

Recombinant Creatine kinase B type Monoclonal Antibody (ET7107-52)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Creatine kinase B type on different lysates using anti-Creatine kinase B type antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Mouse brain <br />
  Lane 2: Mouse brain<br />
  Lane 3: Rat brain <br />
  Lane 4: 293
  • Western blot analysis of Creatine kinase B type on different lysates using anti-Creatine kinase B type antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Mouse brain <br />
  Lane 2: Mouse brain<br />
  Lane 3: Rat brain <br />
  Lane 4: 293
  • ICC staining Creatine kinase B type in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Creatine kinase B type in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Creatine kinase B type antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Creatine kinase B type antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Creatine kinase B type antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of 293T cells with Creatine kinase B type antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Creatine kinase B type on different lysates using anti-Creatine kinase B type antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse brain
Lane 2: Mouse brain
Lane 3: Rat brain
Lane 4: 293

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Creatine kinase B type Monoclonal Antibody (ET7107-52)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat brain tissue, human prostate tissue, mouse colon tissue, mouse brain tissue lysate, 293, 293T, LOVO.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB78-34

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

43 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Creatine kinase B type

SYNONYMS

B CK antibody; B-CK antibody; BB-CK antibody; BCK antibody; Brain creatine kinase antibody; Ckb antibody; CKBB antibody; Creatine kinase B antibody; Creatine kinase B chain antibody; Creatine kinase B type antibody; Creatine kinase B-type antibody; Creatine Kinase BB Isoenzyme antibody; Creatine kinase brain antibody; Creatine kinase brain type antibody; Creatine phosphokinase BB antibody; Epididymis luminal protein 211 antibody; Epididymis secretory protein Li 29 antibody; HEL 211 antibody; HEL S 29 antibody; KCRB_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the ATP:guanido phosphotransferase family.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Creatine kinases (CKs) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. Creatine kinases provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems including muscle, electrocytes, retina photoreceptor cells, brain cells, kidney, salt glands, myometrium, placenta, pancreas, thymus, thyroid, intestinal epithelial cells, endothelial cells, cartilage and bone cells, macrophages, blood platelets, and tumor and cancer cells. Human cytoplasmic creatine kinase-B, also designated CK-B and BCK, is a 381 amino acid, brain tissue specific isoform of creatine kinase. Human cytoplasmic creatine kinase-muscle (CK-M, MCK) is a muscle tissue-specific isoform of creatine kinase. Human cytoplasmic creatine kinase-Mi (Mi-CK, MtCK) is a 416 amino acid mitochondrial-specific isoform of creatine kinase. Cytosolic creatine kinases are important in the energetic regulation of Ca2+-pumps and in the maintenance of Ca2+-homeostasis.