PRODUCT CODE: ET1702-13

Recombinant Collagen X Monoclonal Antibody (ET1702-13)

  • Recombinant

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Collagen X on rat liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-13, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of Collagen X on rat liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-13, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Collagen X on rat liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-13, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Collagen X Monoclonal Antibody (ET1702-13)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat liver tissue lysates.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF0961

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

66 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

Collagen X

SYNONYMS

COAA1_HUMAN antibody; Col10a 1 antibody; COL10A1 antibody; Collagen alpha 1(X) chain antibody; Collagen alpha-1(X) chain antibody; Collagen type X alpha 1 (Schmid metaphyseal chondrodysplasia) antibody; Collagen type X alpha 1 antibody; Collagen X alpha 1 polypeptide antibody; CollagenX antibody; fa66d11 antibody; fb10c08 antibody; OTTHUMP00000040411 antibody; Procollagen type X alpha 1 antibody; Schmid metaphyseal chondrodysplasia antibody; wu:fa66d11 antibody; wu:fb10c08 antibody

POST-TRANSLATIONAL MODIFICATION

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

SUBCELLULAR LOCATION

Extracellular matrix.

FUNCTION

Collagen Type X is associated with hypertrophic chondrocytes of avian and mammalian growth plate tissues during the endochondral growth of long bones. It is a component of normal articular cartilage in adult human, growing pig and newborn rat, and it is also present during any disruption of normal metabolic status of articular cartilage that occur with osteoarthritis. Collagen Type X is composed of three identical a 1(X) chains, each containing a triple-helical region flanked by a short N-terminal sequence and a larger non-collagenous C-terminal (NC1) domain. Mutations in COL10A1, the gene encoding for Collagen Type X, are associated with metaphyseal dysplasia type Schmid (SMCD) and other related forms of metaphyseal dysplasia. SMCD is characterized by short-limbed dwarfism, an outward "flaring" of the lower rib cage, bowed legs, leg pain and a hip deformity that causes the thigh bone to angle toward the center of the body.