Positive control:
Lane 1: human skeletal muscle tissue lysate
Lane 2: mouse heart tissue lysate
Lane 3: human heart tissue lysate
Applications
-
WB
-
ICC
-
IF
-
IHC-P
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant Collagen VI Monoclonal Antibody (ET1612-91)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Human skeletal muscle tissue lysate, mouse heart tissue lysate, human heart tissue lysate, Hela, A549, HepG2, RH-35, human lung tissue, human liver tissue, mouse colon tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SD83-03
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
134/109 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
ICC/IF
-
1:100-1:500
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
Collagen VI
SYNONYMS
Alpha 1 (VI) chain (61 AA) antibody; CO6A1_HUMAN antibody; COL6A1 antibody; COL6A2 antibody; COL6A3 antibody; Collagen alpha 2(VI) chain antibody; Collagen alpha 3(VI) chain antibody; Collagen alpha-1(VI) chain antibody; Collagen type VI alpha 1 antibody; Collagen type VI alpha 2 antibody; Collagen type VI alpha 3 antibody; Collagen VI alpha 1 polypeptide antibody; Collagen VI alpha 2 polypeptide antibody; Collagen VI alpha 3 polypeptide antibody; CollagenVI antibody; Human mRNA for collagen VI alpha 2 C terminal globular domain antibody; OPLL antibody; PP3610 antibody
SEQUENCE SIMILARITIES
Belongs to the type VI collagen family.
POST-TRANSLATIONAL MODIFICATION
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
SUBCELLULAR LOCATION
Extracellular matrix.
FUNCTION
This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.