PRODUCT CODE: ET7106-82

Recombinant Coilin Monoclonal Antibody (ET7106-82)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

Western blot analysis of Coilin on rat kidney tissue lysates using anti-Coilin at 1/500 dilution.
  • Western blot analysis of Coilin on rat kidney tissue lysates using anti-Coilin at 1/500 dilution.
  • Flow cytometric analysis of Jurkat cells with Coilin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Coilin on rat kidney tissue lysates using anti-Coilin at 1/500 dilution.

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Coilin Monoclonal Antibody (ET7106-82)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat kidney tissue lysates, Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU44-08

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

63 kDa (Predicted band size)

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Coilin

SYNONYMS

CLN 80 antibody; CLN80 antibody; COIL antibody; COIL_HUMAN antibody; Coilin antibody; Coilin p80 antibody; p80 antibody; P80 coilin antibody

SEQUENCE SIMILARITIES

Belongs to the coilin family.

TISSUE SPECIFICITY

Found in all the cell types examined.

POST-TRANSLATIONAL MODIFICATION

Symmetrical dimethylation of arginine residues within the RG repeat region enhances affinity for SMN, and thus localization of SMN complexes to CBs.; Phosphorylation during mitosis is associated with disassembly of CBs.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Coilin protein is one of the main molecular components of Cajal bodies (CBs). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. In addition to its structural role, coilin acts as glue to connect the CB to the nucleolus. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome and chromosome.