Positive control:
Lane 1: mouse brain tissue lysate
Lane 2: mouse cerebrum tissue lysate
Applications
-
WB
-
ICC
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant CNPase Monoclonal Antibody (ET1702-46)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Mouse brain tissue lysate, mouse cerebrum tissue lysate, SHG-44, SH-SY5Y, N2A, mouse brain tissue, rat brain tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JF10-25
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
48 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
ICC
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
CNPase
SYNONYMS
2' antibody; 2'3' cyclic nucleotide 3' phosphodiesterase antibody; 3'-cyclic-nucleotide 3'-phosphodiesterase antibody; CN37_HUMAN antibody; CNP 1 antibody; CNP antibody; CNP1 antibody; CNPase antibody
SEQUENCE SIMILARITIES
Belongs to the 2H phosphoesterase superfamily. CNPase family.
SUBCELLULAR LOCATION
Membrane, Melanosome.
FUNCTION
2',3'-cyclic nucleotide-3'-phosphodiesterase (CNPase) is a membrane-bound enzyme that can link tubulin to membranes and may regulate cytoplasmic microtubule distribution. CNPase acts as a microtubule-associated protein by promoting microtubule assembly; this activity resides in the C-terminus of the enzyme. CNPase is firmly associated with tubulin from brain tissue and thyroid cells and can be found at high concentrations in central nervous system myelin and in the outer segments of photoreceptors in the retina. Acute lead intoxication leads to disturbances in CNPase activity and the morphology of myelin.